Next Article in Journal
Supplementation of Antipsychotic Treatment with the Amino Acid Sarcosine Influences Proton Magnetic Resonance Spectroscopy Parameters in Left Frontal White Matter in Patients with Schizophrenia
Previous Article in Journal
The Spectrum of Differences between Childhood and Adulthood Celiac Disease
Article Menu

Export Article

Open AccessArticle
Nutrients 2015, 7(10), 8752-8766; doi:10.3390/nu7105434

Proteomics Analysis to Identify and Characterize the Molecular Signatures of Hepatic Steatosis in Ovariectomized Rats as a Model of Postmenopausal Status

1
Proteomics Research Center, National Yang-Ming University, Taipei 11221, Taiwan
2
Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan 33301, Taiwan
3
Department of Orthopedic Surgery, Taipei Medical University Shuang Ho Hospital, New Taipei City 23561, Taiwan
4
School of Nutrition and Health Sciences, Taipei Medical University, Taipei 11031, Taiwan
5
National Laboratory Animal Center, National Applied Research Laboratories, Taipei 11529, Taiwan
6
Department of Forestry, National Chung Hsing University, Taichung 40227, Taiwan
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 28 August 2015 / Revised: 7 October 2015 / Accepted: 15 October 2015 / Published: 22 October 2015
View Full-Text   |   Download PDF [2167 KB, uploaded 27 October 2015]   |  

Abstract

Postmenopausal women are particularly at increased risk of developing non-alcoholic fatty liver disease (NAFLD). Here we aimed to determine the impact of postmenopausal-induced NAFLD (PM-NAFLD) in an ovariectomized rat model. Sixteen six-week-old Sprague-Dawley female rats were randomly divided into two groups (eight per group), for sham-operation (Sham) or bilateral ovariectomy (Ovx). Four months after surgery, indices of liver damage and liver histomorphometry were measured. Both serum aspartate aminotransferase (AST) and alanine aminotranferease (ALT) levels were significantly higher in the Ovx than Sham group. We performed quantitative LC-MS/MS-based proteomic profiling of livers from rats with PM-NAFLD to provide baseline knowledge of the PM-NAFLD proteome and to investigate proteins involved in PM-NAFLD by ingenuity pathways analysis (IPA) to provide corroborative evidence for differential regulation of molecular and cellular functions affecting metabolic processes. Of the 586 identified proteins, the levels of 59 (10.0%) and 48 (8.2%) were significantly higher and lower, respectively, in the Ovx group compared to the Sham group. In conclusion, the changes in regulation of proteins implicated in PM-NAFLD may affect other vital biological processes in the body apart from causing postmenopause-mediated liver dysfunction. Our quantitative proteomics analysis may also suggest potential biomarkers and further clinical applications for PM-NAFLD. View Full-Text
Keywords: liver; proteome; ingenuity pathways analysis liver; proteome; ingenuity pathways analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Liao, C.-C.; Chiu, Y.-S.; Chiu, W.-C.; Tung, Y.-T.; Chuang, H.-L.; Wu, J.-H.; Huang, C.-C. Proteomics Analysis to Identify and Characterize the Molecular Signatures of Hepatic Steatosis in Ovariectomized Rats as a Model of Postmenopausal Status. Nutrients 2015, 7, 8752-8766.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top