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Nutrients 2015, 7(10), 8670-8684; doi:10.3390/nu7105423

The Ethanol Extract from Lonicera japonica Thunb. Regresses Nonalcoholic Steatohepatitis in a Methionine- and Choline-Deficient Diet-Fed Animal Model

1
Department of Pharmacy and Master Program, Tajen University, Pingtung, 90741, Taiwan
2
St. Dominic’s Catholic High School, Kaohsiung, 80288, Taiwan
*
Author to whom correspondence should be addressed.
Received: 22 August 2015 / Revised: 19 September 2015 / Accepted: 6 October 2015 / Published: 21 October 2015
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Abstract

Nonalcoholic steatohepatitis (NASH) is characterized as fat accumulation in the hepatic tissue associated with various degrees of inflammation and progressive fibrosis. The potent anti-inflammatory and ethnopharmacological properties of Lonicera japonica Thunb. (Caprifoliaceae) make it an excellent source of novel medicinal targets for the treatment of NASH. The aim of the study was to investigate the effects of L. japonica ethanol extract (LJEE) on NASH in mice. C57BL/6J mice were fed with methionine-choline-deficient diet (MCDD) for eight weeks to promote the development of NASH. After development of the model, the mice were administered LJEE once daily via oral gavage at doses of 100, 200, or 300 mg/kg for another four weeks. Simultaneous treatments with LJEE (300 mg/kg/day) resulted in pronounced improvements in liver steatosis, ballooning degeneration, and inflammation. LJEE prevented MCDD-induced plasma level increases in aspartate aminotransferase and alanine aminotransferase. LJEE significantly reduced hepatic malondialdehyde level and ameliorated hepatic inflammation and fibrosis in MCDD-fed mice, which were associated with down-regulation of cytochrome P450 2E1 suppression of multiple proinflammatory and profibrotic genes. LJEE can prevent hepatic steatosis by reducing hepatic peroxisome acyl-CoA:diacylglycerol acyltransferase 2 expression, as well as by inducing proliferator-activated receptor α expression. In addition, the LJEE treatments caused significant reduction in the phosphorylated form of Jun N-terminal kinase along with an increase in the phosphorylated level of extra cellular signal-regulated kinase 1/2. Our study demonstrated the protective role of LJEE in ameliorating nutritional steatohepatitis. View Full-Text
Keywords: non-alcoholic steatohepatitis (NASH); Lonicera japonica Thunb; methionine; and choline-deficient diet (MCDD); Jun N-terminal kinase; extra cellular signal-regulated kinase 1/2 non-alcoholic steatohepatitis (NASH); Lonicera japonica Thunb; methionine; and choline-deficient diet (MCDD); Jun N-terminal kinase; extra cellular signal-regulated kinase 1/2
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tzeng, T.-F.; Tzeng, Y.-C.; Cheng, Y.-J.; Liou, S.-S.; Liu, I.-M. The Ethanol Extract from Lonicera japonica Thunb. Regresses Nonalcoholic Steatohepatitis in a Methionine- and Choline-Deficient Diet-Fed Animal Model. Nutrients 2015, 7, 8670-8684.

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