Nutrients 2014, 6(2), 564-574; doi:10.3390/nu6020564
Article

Protective Effect of Wheat Peptides against Indomethacin-Induced Oxidative Stress in IEC-6 Cells

Received: 30 September 2013; in revised form: 23 December 2013 / Accepted: 10 January 2014 / Published: 29 January 2014
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Recent studies have demonstrated that wheat peptides protected rats against non-steroidal anti-inflammatory drugs-induced small intestinal epithelial cells damage, but the mechanism of action is unclear. In the present study, an indomethacin-induced oxidative stress model was used to investigate the effect of wheat peptides on the nuclear factor-κB(NF-κB)-inducible nitric oxide synthase-nitric oxide signal pathway in intestinal epithelial cells-6 cells. IEC-6 cells were treated with wheat peptides (0, 125, 500 and 2000 mg/L) for 24 h, followed by 90 mg/L indomethacin for 12 h. Wheat peptides significantly attenuated the indomethacin-induced decrease in superoxide dismutase and glutathione peroxidase activity. Wheat peptides at 2000 mg/L markedly decreased the expression of the NF-κB in response to indomethacin-induced oxidative stress. This study demonstrated that the addition of wheat peptides to a culture medium significantly inhibited the indomethacin-induced release of malondialdehyde and nitrogen monoxide, and increased antioxidant enzyme activity in IEC-6 cells, thereby providing a possible explanation for the protective effect proposed for wheat peptides in the prevention of indomethacin-induced oxidative stress in small intestinal epithelial cells.
Keywords: wheat peptides; indomethacin; IEC-6 cells; oxidative stress; NF-κB-iNOS-NO signal pathway
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MDPI and ACS Style

Yin, H.; Pan, X.; Song, Z.; Wang, S.; Yang, L.; Sun, G. Protective Effect of Wheat Peptides against Indomethacin-Induced Oxidative Stress in IEC-6 Cells. Nutrients 2014, 6, 564-574.

AMA Style

Yin H, Pan X, Song Z, Wang S, Yang L, Sun G. Protective Effect of Wheat Peptides against Indomethacin-Induced Oxidative Stress in IEC-6 Cells. Nutrients. 2014; 6(2):564-574.

Chicago/Turabian Style

Yin, Hong; Pan, Xingchang; Song, Zhixiu; Wang, Shaokang; Yang, Ligang; Sun, Guiju. 2014. "Protective Effect of Wheat Peptides against Indomethacin-Induced Oxidative Stress in IEC-6 Cells." Nutrients 6, no. 2: 564-574.

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