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Protective Effect of Wheat Peptides against Indomethacin-Induced Oxidative Stress in IEC-6 Cells
Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China
China National Research Institute of Food & Fermentation Industries, Beijing 100028, China
Second School of Clinical Medical, Nanjing University of Chinese Medicine, Nanjing 210023, China
* Author to whom correspondence should be addressed.
Received: 30 September 2013; in revised form: 23 December 2013 / Accepted: 10 January 2014 / Published: 29 January 2014
Abstract: Recent studies have demonstrated that wheat peptides protected rats against non-steroidal anti-inflammatory drugs-induced small intestinal epithelial cells damage, but the mechanism of action is unclear. In the present study, an indomethacin-induced oxidative stress model was used to investigate the effect of wheat peptides on the nuclear factor-κB(NF-κB)-inducible nitric oxide synthase-nitric oxide signal pathway in intestinal epithelial cells-6 cells. IEC-6 cells were treated with wheat peptides (0, 125, 500 and 2000 mg/L) for 24 h, followed by 90 mg/L indomethacin for 12 h. Wheat peptides significantly attenuated the indomethacin-induced decrease in superoxide dismutase and glutathione peroxidase activity. Wheat peptides at 2000 mg/L markedly decreased the expression of the NF-κB in response to indomethacin-induced oxidative stress. This study demonstrated that the addition of wheat peptides to a culture medium significantly inhibited the indomethacin-induced release of malondialdehyde and nitrogen monoxide, and increased antioxidant enzyme activity in IEC-6 cells, thereby providing a possible explanation for the protective effect proposed for wheat peptides in the prevention of indomethacin-induced oxidative stress in small intestinal epithelial cells.
Keywords: wheat peptides; indomethacin; IEC-6 cells; oxidative stress; NF-κB-iNOS-NO signal pathway
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Cite This Article
MDPI and ACS Style
Yin, H.; Pan, X.; Song, Z.; Wang, S.; Yang, L.; Sun, G. Protective Effect of Wheat Peptides against Indomethacin-Induced Oxidative Stress in IEC-6 Cells. Nutrients 2014, 6, 564-574.
Yin H, Pan X, Song Z, Wang S, Yang L, Sun G. Protective Effect of Wheat Peptides against Indomethacin-Induced Oxidative Stress in IEC-6 Cells. Nutrients. 2014; 6(2):564-574.
Yin, Hong; Pan, Xingchang; Song, Zhixiu; Wang, Shaokang; Yang, Ligang; Sun, Guiju. 2014. "Protective Effect of Wheat Peptides against Indomethacin-Induced Oxidative Stress in IEC-6 Cells." Nutrients 6, no. 2: 564-574.