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Nutrients 2014, 6(2), 564-574; doi:10.3390/nu6020564
Article

Protective Effect of Wheat Peptides against Indomethacin-Induced Oxidative Stress in IEC-6 Cells

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1 Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China 2 China National Research Institute of Food & Fermentation Industries, Beijing 100028, China 3 Second School of Clinical Medical, Nanjing University of Chinese Medicine, Nanjing 210023, China
* Author to whom correspondence should be addressed.
Received: 30 September 2013 / Revised: 23 December 2013 / Accepted: 10 January 2014 / Published: 29 January 2014
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Abstract

Recent studies have demonstrated that wheat peptides protected rats against non-steroidal anti-inflammatory drugs-induced small intestinal epithelial cells damage, but the mechanism of action is unclear. In the present study, an indomethacin-induced oxidative stress model was used to investigate the effect of wheat peptides on the nuclear factor-κB(NF-κB)-inducible nitric oxide synthase-nitric oxide signal pathway in intestinal epithelial cells-6 cells. IEC-6 cells were treated with wheat peptides (0, 125, 500 and 2000 mg/L) for 24 h, followed by 90 mg/L indomethacin for 12 h. Wheat peptides significantly attenuated the indomethacin-induced decrease in superoxide dismutase and glutathione peroxidase activity. Wheat peptides at 2000 mg/L markedly decreased the expression of the NF-κB in response to indomethacin-induced oxidative stress. This study demonstrated that the addition of wheat peptides to a culture medium significantly inhibited the indomethacin-induced release of malondialdehyde and nitrogen monoxide, and increased antioxidant enzyme activity in IEC-6 cells, thereby providing a possible explanation for the protective effect proposed for wheat peptides in the prevention of indomethacin-induced oxidative stress in small intestinal epithelial cells.
Keywords: wheat peptides; indomethacin; IEC-6 cells; oxidative stress; NF-κB-iNOS-NO signal pathway wheat peptides; indomethacin; IEC-6 cells; oxidative stress; NF-κB-iNOS-NO signal pathway
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Yin, H.; Pan, X.; Song, Z.; Wang, S.; Yang, L.; Sun, G. Protective Effect of Wheat Peptides against Indomethacin-Induced Oxidative Stress in IEC-6 Cells. Nutrients 2014, 6, 564-574.

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