Nutrients 2013, 5(10), 3898-3909; doi:10.3390/nu5103898
Article

The Role of Vitamin D Level and Related Single Nucleotide Polymorphisms in Crohn’s Disease

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Received: 24 June 2013; in revised form: 19 August 2013 / Accepted: 12 September 2013 / Published: 27 September 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: New Zealand has one of the highest rates of Crohn’s Disease (CD) in the world, and there is much speculation as to why this might be. A high risk of CD has been associated with deficient or insufficient levels of Vitamin D (Vit D), lifestyle as well as various genetic polymorphisms. In this study we sought to analyse the relevance of serum Vit D levels, lifestyle and genotype to CD status. Serum samples were analysed for 25-OH-Vitamin D levels. DNA was isolated from blood and cheek-swabs, and Sequenom and ImmunoChip techniques were used for genotyping. Serum Vit D levels were significantly lower in CD patients (mean = 49.5 mg/L) than those found in controls (mean = 58.9 mg/L, p = 4.74 × 10−6). A total of seven single nucleotide polymorphisms were examined for effects on serum Vit D levels, with adjustment for confounding variables. Two variants: rs731236[A] (VDR) and rs732594[A] (SCUBE3) showed a significant association with serum Vit D levels in CD patients. Four variants: rs7975232[A] (VDR), rs732594[A] (SCUBE3), and rs2980[T] and rs2981[A] (PHF-11) showed a significant association with serum Vit D levels in the control group. This study demonstrates a significant interaction between Vit D levels and CD susceptibility, as well as a significant association between Vit D levels and genotype.
Keywords: vitamin D levels; Crohn’s disease; VDR; SCUBE3; PPP6R3; PHF-11; SNPs; lifestyle
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MDPI and ACS Style

Carvalho, A.Y.O.M.; Bishop, K.S.; Han, D.Y.; Ellett, S.; Jesuthasan, A.; Lam, W.J.; Ferguson, L.R. The Role of Vitamin D Level and Related Single Nucleotide Polymorphisms in Crohn’s Disease. Nutrients 2013, 5, 3898-3909.

AMA Style

Carvalho AYOM, Bishop KS, Han DY, Ellett S, Jesuthasan A, Lam WJ, Ferguson LR. The Role of Vitamin D Level and Related Single Nucleotide Polymorphisms in Crohn’s Disease. Nutrients. 2013; 5(10):3898-3909.

Chicago/Turabian Style

Carvalho, Andre Y.O.M.; Bishop, Karen S.; Han, Dug Y.; Ellett, Stephanie; Jesuthasan, Amalini; Lam, Wen J.; Ferguson, Lynnette R. 2013. "The Role of Vitamin D Level and Related Single Nucleotide Polymorphisms in Crohn’s Disease." Nutrients 5, no. 10: 3898-3909.

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