Orosensory Detection of Dietary Fatty Acids Is Altered in CB1R−/− Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethical Approval
2.2. Behavioral Experiments
2.2.1. Two-Bottle Preference Tests
2.2.2. Licking Tests
2.3. Papillae and Taste Buds Isolation
2.4. Real-Time qPCR
2.5. Western Blotting
2.6. Tissue Culture of TBC and GLP-1 Release
2.7. Measurement of Ca2+ Signaling
2.8. Statistics
3. Results
3.1. The Absence of CB1R Gene Induces a Low Preference for Fatty Solutions Independently of Postprandial Factors
3.2. Treatment with Rimonabant Induces a Low Preference for Fat Solutions and Does Not Alter Feeding Behavior
3.3. CD36 and GPR120 Protein Expressions Are Not Altered in TBC of CB1R−/− Mice
3.4. CB1R Gene Invalidation Induces a Decrease in Proglucagon and GLP-1r mRNA and Basal GLP-1 Level
3.5. Both LA and Cannabinoids Induce CB1R-Dependent Ca2+ Responses in TBC
3.6. CB1R Blockade Significantly Decreases Ca2+ Responses Triggered by LA, AEA, and ACEA in WT TBC
3.7. AEA-Induced Ca2+-Signaling Is PLC Dependent
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Brissard, L.; Leemput, J.; Hichami, A.; Passilly-Degrace, P.; Maquart, G.; Demizieux, L.; Degrace, P.; Khan, N.A. Orosensory Detection of Dietary Fatty Acids Is Altered in CB1R−/− Mice. Nutrients 2018, 10, 1347. https://doi.org/10.3390/nu10101347
Brissard L, Leemput J, Hichami A, Passilly-Degrace P, Maquart G, Demizieux L, Degrace P, Khan NA. Orosensory Detection of Dietary Fatty Acids Is Altered in CB1R−/− Mice. Nutrients. 2018; 10(10):1347. https://doi.org/10.3390/nu10101347
Chicago/Turabian StyleBrissard, Léa, Julia Leemput, Aziz Hichami, Patricia Passilly-Degrace, Guillaume Maquart, Laurent Demizieux, Pascal Degrace, and Naim Akhtar Khan. 2018. "Orosensory Detection of Dietary Fatty Acids Is Altered in CB1R−/− Mice" Nutrients 10, no. 10: 1347. https://doi.org/10.3390/nu10101347