An 8-Week, Low Carbohydrate, High Fat, Ketogenic Diet Enhanced Exhaustive Exercise Capacity in Mice Part 2: Effect on Fatigue Recovery, Post-Exercise Biomarkers and Anti-Oxidation Capacity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mouse Maintenance and Diets
2.2. Endurance Capacity Test Protocol
2.3. Open Field Analysis
2.4. Plasma Biochemical Assessment
2.5. Lactate Assay
2.6. Measurement of Oxidative Stress
2.7. Statistical Analysis
3. Results and Discussion
3.1. KD Lowered Mice Weight
3.2. KD Accelerated Fatigue Recovery after a 24-h Rest
3.3. Plasma Substrate Concentrations Were Altered by KD after a 24-h rest
3.4. KD Contributed to an Accelarated Damage Recovery
3.5. TBARs and Protein Carbonyl Level Is Not Alternated by KD
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
- Suzuki, K.; Totsuka, M.; Nakaji, S.; Yamada, M.; Kudoh, S.; Liu, Q.; Sugawara, K.; Yamaya, K.; Sato, K. Endurance exercise causes interaction among stress hormones, cytokines, neutrophil dynamics, and muscle damage. J. Appl. Physiol. 1999, 87, 1360–1367. [Google Scholar] [CrossRef] [PubMed]
- Suzuki, K. Cytokine Response to Exercise and Its Modulation. Antioxidants 2018, 7, 17. [Google Scholar] [CrossRef]
- Suzuki, K. Exhaustive Exercise-Induced Neutrophil-Associated Tissue Damage and Possibility of its Prevention. J. Nanomed. Biother. Discov. 2017, 7, 156. [Google Scholar] [CrossRef]
- Huang, W.C.; Lin, C.I.; Chiu, C.C.; Lin, Y.T.; Huang, W.K.; Huang, H.Y.; Huang, C.C. Chicken essence improves exercise performance and ameliorates physical fatigue. Nutrients 2014, 6, 2681–2696. [Google Scholar] [CrossRef] [PubMed]
- Chen, Y.M.; Tsai, Y.H.; Tsai, T.Y.; Chiu, Y.S.; Wei, L.; Chen, W.C.; Huang, C.C. Fucoidan supplementation improves exercise performance and exhibits anti-fatigue action in mice. Nutrients 2014, 7, 239–252. [Google Scholar] [CrossRef] [PubMed]
- Kim, H.J.; Lee, Y.H.; Kim, C.K. Biomarkers of muscle and cartilage damage and inflammation during a 200 km run. Eur. J. Appl. Physiol. 2007, 99, 443–447. [Google Scholar] [CrossRef] [PubMed]
- Wu, H.J.; Chen, K.T.; Shee, B.W.; Chang, H.C.; Huang, Y.J.; Yang, R.S. Effects of 24 h ultra-marathon on biochemical and hematological parameters. World J. Gastroenterol. 2004, 10, 2711–2714. [Google Scholar] [CrossRef] [PubMed]
- Chen, Y.M.; Wei, L.; Chiu, Y.S.; Hsu, Y.J.; Tsai, T.Y.; Wang, M.F.; Huang, C.C. Lactobacillus plantarum TWK10 supplementation improves exercise performance and increases muscle mass in mice. Nutrients 2016, 8, 205. [Google Scholar] [CrossRef] [PubMed]
- Tipton, K.D. Nutritional support for exercise-induced injuries. Sports Med. 2015, 45, 93–104. [Google Scholar] [CrossRef] [PubMed]
- Ma, S.; Huang, Q.; Yada, K.; Liu, C.; Suzuki, K. An 8-Week Ketogenic Low Carbohydrate, High Fat Diet Enhanced Exhaustive Exercise Capacity in Mice. Nutrients 2018, 10, 673. [Google Scholar] [CrossRef] [PubMed]
- Kephart, W.C.; Mumford, P.W.; Mao, X.; Romero, M.A.; Hyatt, H.W.; Zhang, Y.; Mobley, C.B.; Quindry, J.C.; Young, K.C.; Beck, D.T.; et al. The 1-week and 8-month effects of a ketogenic diet or ketone salt supplementation on multi-organ markers of oxidative stress and mitochondrial function in rats. Nutrients 2017, 9, 1019. [Google Scholar] [CrossRef] [PubMed]
- Cairns, S.P. Lactic acid and exercise performance. Sports Med. 2006, 36, 279–291. [Google Scholar] [CrossRef] [PubMed]
- Allen, D.G.; Lamb, G.D.; Westerblad, H. Skeletal muscle fatigue: Cellular mechanisms. Physiol. Rev. 2008, 88, 287–332. [Google Scholar] [CrossRef] [PubMed]
- Ahlborg, B.; Bergström, J.; Ekelund, L.G.; Hultman, E. Muscle glycogen and muscle electrolytes during prolonged physical exercise1. Acta Physiol. Scand. 1967, 70, 129–142. [Google Scholar] [CrossRef]
- Karlsson, J.; Saltin, B. Diet, muscle glycogen, and endurance performance. J. Appl. Physiol. 1971, 31, 203–206. [Google Scholar] [CrossRef] [PubMed]
- Powers, S.K.; Jackson, M.J. Exercise-induced oxidative stress: Cellular mechanisms and impact on muscle force production. Physiol. Rev. 2008, 88, 1243–1276. [Google Scholar] [CrossRef] [PubMed]
- Ogonovszky, H.; Berkes, I.; Kumagai, S.; Kaneko, T.; Tahara, S.; Goto, S.; Radák, Z. The effects of moderate-, strenuous-and over-training on oxidative stress markers, DNA repair, and memory, in rat brain. Neurochem. Int. 2005, 46, 635–640. [Google Scholar] [CrossRef] [PubMed]
- Radak, Z.; Chung, H.Y.; Koltai, E.; Taylor, A.W.; Goto, S. Exercise, oxidative stress and hormesis. Ageing Res. Rev. 2008, 7, 34–42. [Google Scholar] [CrossRef] [PubMed]
- Radak, Z.; Asano, K.; Inoue, M.; Kizaki, T.; Oh-Ishi, S.; Suzuki, K.; Taniguchi, N.; Ohno, H. Superoxide dismutase derivative reduces oxidative damage in skeletal muscle of rats during exhaustive exercise. J. Appl. Physiol. 1995, 79, 129–135. [Google Scholar] [CrossRef] [PubMed]
- Murase, T.; Haramizu, S.; Shimotoyodome, A.; Tokimitsu, I.; Hase, T. Green tea extract improves running endurance in mice by stimulating lipid utilization during exercise. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2006, 290, R1550–R1556. [Google Scholar] [CrossRef] [PubMed]
- Gao, L.; Rudebush, T.L.; Wafi, A.M.; Zucker, I.H.; Schultz, H.D. Nrf2 Activation by Curcumin Improves Exercise Performance of Mice with Chronic Heart Failure. FASEB J. 2017, 31 (Suppl. 1), 1020.15. [Google Scholar]
- Matuszczak, Y.; Farid, M.; Jones, J.; Lansdowne, S.; Smith, M.A.; Taylor, A.A.; Reid, M.B. Effects of N-acetylcysteine on glutathione oxidation and fatigue during handgrip exercise. Muscle Nerve 2005, 32, 633–638. [Google Scholar] [CrossRef] [PubMed]
- McKenna, M.J.; Medved, I.; Goodman, C.A.; Brown, M.J.; Bjorksten, A.R.; Murphy, K.T.; Peterson, A.C.; Sostaric, S.; Gong, X. N-acetylcysteine attenuates the decline in muscle Na+, K+-pump activity and delays fatigue during prolonged exercise in humans. J. Physiol. 2006, 576, 279–288. [Google Scholar] [CrossRef] [PubMed]
- Basso, D.M.; Beattie, M.S.; Bresnahan, J.C. A sensitive and reliable locomotor rating scale for open field testing in rats. J. Neurotrauma 1995, 12, 1–21. [Google Scholar] [CrossRef] [PubMed]
- Basso, D.M.; Beattie, M.S.; Bresnahan, J.C.; Anderson, D.K.; Faden, A.I.; Gruner, J.A.; Holford, T.R.; Hsu, Y.; Noble, J.; Nockels, R.; et al. MASCIS evaluation of open field locomotor scores: Effects of experience and teamwork on reliability. J. Neurotrauma 1996, 13, 343–359. [Google Scholar] [CrossRef] [PubMed]
- Davis, J.M.; Zhao, Z.; Stock, H.S.; Mehl, K.A.; Buggy, J.; Hand, G.A. Central nervous system effects of caffeine and adenosine on fatigue. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2003, 284, R399–R404. [Google Scholar] [CrossRef] [PubMed]
- Paoli, A.; Rubini, A.; Volek, J.S.; Grimaldi, K.A. Beyond weight loss: A review of the therapeutic uses of very-low-carbohydrate (ketogenic) diets. Eur. J. Clin. Nutr. 2013, 67, 789. [Google Scholar] [CrossRef] [PubMed]
- Newman, J.C.; Covarrubias, A.J.; Zhao, M.; Yu, X.; Gut, P.; Ng, C.P.; Huang, Y.; Halder, S.; Verdin, E. Ketogenic diet reduces midlife mortality and improves memory in aging mice. Cell Metab. 2017, 26, 547–557. [Google Scholar] [CrossRef] [PubMed]
- Samaha, F.F.; Iqbal, N.; Seshadri, P.; Chicano, K.L.; Daily, D.A.; McGrory, J.; Williams, T.; Williams, M.; Gracely, E.J.; Stern, L. A low-carbohydrate as compared with a low-fat diet in severe obesity. N. Engl. J. Med. 2003, 348, 2074–2081. [Google Scholar] [CrossRef] [PubMed]
- Stern, L.; Iqbal, N.; Seshadri, P.; Chicano, K.L.; Daily, D.A.; McGrory, J.; Williams, M.; Gracely, E.J.; Samaha, F.F. The effects of low-carbohydrate versus conventional weight loss diets in severely obese adults: One-year follow-up of a randomized trial. Ann. Intern. Med. 2004, 140, 778–785. [Google Scholar] [CrossRef] [PubMed]
- Gibson, A.A.; Seimon, R.V.; Lee, C.M.; Ayre, J.; Franklin, J.; Markovic, T.P.; Caterson, I.D.; Sainsbury, A. Do ketogenic diets really suppress appetite? A systematic review and meta-analysis. Obes. Rev. 2015, 16, 64–76. [Google Scholar] [CrossRef] [PubMed]
- McAuley, P.A.; Keteyian, S.J.; Brawner, C.A.; Dardari, Z.A.; Al Rifai, M.; Ehrman, J.K.; Mouaz, H.A.; Whelton, S.P.; Blaha, M.J. Exercise capacity and the obesity paradox in heart failure: The FIT (Henry Ford Exercise Testing) Project. Mayo Clin. Proc. 2018, 93, 701–708. [Google Scholar] [CrossRef] [PubMed]
- Seibenhener, M.L.; Wooten, M.C. Use of the open field maze to measure locomotor and anxiety-like behavior in mice. J. Visual. Exp. 2015, 96, 52434. [Google Scholar] [CrossRef] [PubMed]
- Phinney, S.D.; Bistrian, B.R.; Evans, W.J.; Gervino, E.; Blackburn, G.L. The human metabolic response to chronic ketosis without caloric restriction: Preservation of submaximal exercise capability with reduced carbohydrate oxidation. Metabolism 1983, 32, 769–776. [Google Scholar] [CrossRef]
- Batson, G. Exercise-induced central fatigue: A review of the literature with implications for dance science research. J. Dance Med. Sci. 2013, 17, 53–62. [Google Scholar] [CrossRef] [PubMed]
- Yoshida, T.; Chida, M.; Ichioka, M.; Suda, Y. Blood lactate parameters related to aerobic capacity and endurance performance. Eur. J. Appl. Physiol. 1987, 56, 7–11. [Google Scholar] [CrossRef]
- Billat, V.L.; Sirvent, P.; Py, G.; Koralsztein, J.P.; Mercier, J. The concept of maximal lactate steady state. Sports Med. 2003, 33, 407–426. [Google Scholar] [CrossRef] [PubMed]
- Martin, N.A.; Zoeller, R.F.; Robertson, R.J.; Lephart, S.M. The comparative effects of sports massage, active recovery, and rest in promoting blood lactate clearance after supramaximal leg exercise. J. Athl. Train. 1998, 33, 30. [Google Scholar] [PubMed]
- Menzies, P.; Menzies, C.; McIntyre, L.; Paterson, P.; Wilson, J.; Kemi, O.J. Blood lactate clearance during active recovery after an intense running bout depends on the intensity of the active recovery. J. Sports Sci. 2010, 28, 975–982. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Mutch, B.J.C.; Banister, E.W. Ammonia metabolism in exercise and fatigue: A review. Med. Sci. Sports Exerc. 1983, 15, 41–50. [Google Scholar] [CrossRef] [PubMed]
- Narasimhan, L.R.; Goodman, W.; Patel, C.K.N. Correlation of breath ammonia with blood urea nitrogen and creatinine during hemodialysis. Proc. Natl. Acad. Sci. USA 2001, 98, 4617–4621. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Zhang, Z.; Xu, X. Lactate clearance is a useful biomarker for the prediction of all-cause mortality in critically ill patients: A systematic review and meta-analysis. Crit. Care Med. 2014, 42, 2118–2125. [Google Scholar] [CrossRef] [PubMed]
- Pösö, A.R.; Viljanen-Tarifa, E.; Soveri, T.; Oksanen, H.E. Exercise-induced transient hyperlipidemia in the racehorse. J. Vet. Med. A Physiol. Pathol. Clin. Med. 1989, 36, 603–611. [Google Scholar] [CrossRef]
- Schnyder, S.; Svensson, K.; Cardel, B.; Handschin, C. Muscle PGC-1α is required for long-term systemic and local adaptations to a ketogenic diet in mice. Am. J. Physiol. Endocrinol. Metab. 2017, 312, E437–E446. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Vranceanu, M.; Grimaldi, K.A.; Perricone, M.; Filip, L. Long term effects of a ketogenic diet with MaVketoFast pro supplement on blood glucose, triglycerides, cholesterol, waist circumference and weight control in obese postmenopausal women. Surg. Obes. Relat. Dis. 2017, 13, S200. [Google Scholar] [CrossRef]
- Mann, S.; Beedie, C.; Jimenez, A. Differential effects of aerobic exercise, resistance training and combined exercise modalities on cholesterol and the lipid profile: Review, synthesis and recommendations. Sports Med. 2014, 44, 211–221. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ambery, A.G.; Tackett, L.; Penque, B.A.; Brozinick, J.T.; Elmendorf, J.S. Exercise training prevents skeletal muscle plasma membrane cholesterol accumulation, cortical actin filament loss, and insulin resistance in C57BL/6J mice fed a western-style high-fat diet. Physiol. Rep. 2017, 5, e13363. [Google Scholar] [CrossRef] [PubMed]
- Suzuki, K.; Nakaji, S.; Yamada, M.; Liu, Q.; Kurakake, S.; Okamura, N.; Kumae, T.; Umeda, T.; Sugawara, K. Impact of a competitive marathon race on systemic cytokine and neutrophil responses. Med. Sci. Sports Exerc. 2003, 35, 348–355. [Google Scholar] [CrossRef] [PubMed]
- Monteiro, M.E.L.; Xavier, A.R.; Azeredo, V.B. Diet and liver apoptosis in rats: A particular metabolic pathway. Nutr. Hosp. 2017, 34, 463–468. [Google Scholar] [CrossRef] [PubMed]
- Zhang, X.; Qin, J.; Zhao, Y.; Shi, J.; Lan, R.; Gan, Y.; Ren, H.; Zhu, B.; Qian, M.; Du, B. Long-term ketogenic diet contributes to glycemic control but promotes lipid accumulation and hepatic steatosis in type 2 diabetic mice. Nutr. Res. 2016, 36, 349–358. [Google Scholar] [CrossRef] [PubMed]
- Davies, K.J.; Quintanilha, A.T.; Brooks, G.A.; Packer, L. Free radicals and tissue damage produced by exercise. Biochem. Biophys. Res. Commun. 1982, 107, 1198–1205. [Google Scholar] [CrossRef]
- Poli, G. Liver damage due to free radicals. Br. Med. Bull. 1993, 49, 604–620. [Google Scholar] [CrossRef] [PubMed]
- Pearcey, G.E.; Bradbury-Squires, D.J.; Kawamoto, J.E.; Drinkwater, E.J.; Behm, D.G.; Button, D.C. Foam rolling for delayed-onset muscle soreness and recovery of dynamic performance measures. J. Athl. Train. 2015, 50, 5–13. [Google Scholar] [CrossRef] [PubMed]
- Clifford, T.; Berntzen, B.; Davison, G.W.; West, D.J.; Howatson, G.; Stevenson, E.J. Effects of beetroot juice on recovery of muscle function and performance between bouts of repeated sprint exercise. Nutrients 2016, 8, 506. [Google Scholar] [CrossRef] [PubMed]
- Elman, R.; Arneson, N.; Graham, E.A. Value of blood amylase estimations in the diagnosis of pancreatic disease: A clinical study. Arch. Surg. 1929, 19, 943–967. [Google Scholar] [CrossRef]
- Janowitz, H.D.; Dreiling, D.A. The plasma amylase: Source, regulation and diagnostic significance. Am. J. Med. 1959, 27, 924–935. [Google Scholar] [CrossRef]
- Pearson, T.; Wattis, J.A.; King, J.R.; MacDonald, I.A.; Mazzatti, D.J. The effects of insulin resistance on individual tissues: An application of a mathematical model of metabolism in humans. Bull. Math. Biol. 2016, 78, 1189–1217. [Google Scholar] [CrossRef] [PubMed]
- Liśkiewicz, A.D.; Kasprowska, D.; Wojakowska, A.; Polański, K.; Lewin-Kowalik, J.; Kotulska, K.; Jędrzejowska-Szypułka, H. Long-term high fat ketogenic diet promotes renal tumor growth in a rat model of tuberous sclerosis. Sci. Rep. 2016, 6, 21807. [Google Scholar] [CrossRef] [PubMed]
- Yi, W.; Xie, X.; Du, M.; Bu, Y.; Wu, N.; Yang, H.; Tian, C.; Xu, F.; Xiang, S.; Zhang, P.; et al. Green tea polyphenols ameliorate the early renal damage induced by a high-fat diet via ketogenesis/SIRT3 pathway. Oxid. Med. Cell. Longev. 2017, 2017, 9032792. [Google Scholar] [CrossRef] [PubMed]
- Carmichael, M.D.; Davis, J.M.; Murphy, E.A.; Brown, A.S.; Carson, J.A.; Mayer, E.P.; Ghaffar, A. Role of brain IL-1β on fatigue after exercise-induced muscle damage. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2006, 291, R1344–R1348. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Starnes, J.; Herberg, T.; Lee, K.T.; Hixson, L.; Vervaecke, L.; Goldfarb, A. Effect of Core Temperature during Exercise on Markers of Muscle Injury and Oxidative Stress. FASEB J. 2017, 31 (Suppl. 1), 1023.3. [Google Scholar]
- Afzalpour, M.E.; Bashafaat, H.; Shariat, A.; Sadeghi, H.; Shaw, I.; Dashtiyan, A.A.; Shaw, B.S. Plasma protein carbonyl responses to anaerobic exercise in female cyclists. Int. J. Appl. Exerc. Physiol. 2016, 5, 53–58. [Google Scholar]
- Duranti, G.; Ceci, R.; Patrizio, F.; Sgrò, P.; Di Luigi, L.; Sabatini, S.; Felici, F.; Bazzucchi, I. Chronic consumption of quercetin reduces erythrocytes oxidative damage: Evaluation at resting and after eccentric exercise in humans. Nutr. Res. 2018, 50, 73–81. [Google Scholar] [CrossRef] [PubMed]
- Morillas-Ruiz, J.M.; García, J.V.; López, F.J.; Vidal-Guevara, M.L.; Zafrilla, P. Effects of polyphenolic antioxidants on exercise-induced oxidative stress. Clin. Nutr. 2006, 25, 444–453. [Google Scholar] [CrossRef] [PubMed]
- Milder, J.; Patel, M. Modulation of oxidative stress and mitochondrial function by the ketogenic diet. J. Epilepsy Res. 2012, 100, 295–303. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jarrett, S.G.; Milder, J.B.; Liang, L.P.; Patel, M. The ketogenic diet increases mitochondrial glutathione levels. J. Neurochem. 2008, 106, 1044–1051. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Milder, J.B.; Liang, L.P.; Patel, M. Acute oxidative stress and systemic Nrf2 activation by the ketogenic diet. Neurobiol. Dis. 2010, 40, 238–244. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Parry, H.A.; Kephart, W.; Mumford, P.W.; Romero, M.A.; Hann, C.; Mobley, C.B.; Zhang, Y.; Roberts, M.D.; Kavazis, A.N. Lifelong Ketogenic Diet Feeding Increases Longevity, But Does Not Alter Oxidative Stress Markers in Rats. Med. Sci. Sports Exerc. 2018, 50, 82. [Google Scholar] [CrossRef]
- Nemes, R.; Koltai, E.; Taylor, A.W.; Suzuki, K.; Gyori, F.; Radak, Z. Reactive Oxygen and Nitrogen Species Regulate Key Metabolic, Anabolic, and Catabolic Pathways in Skeletal Muscle. Antioxidants 2018, 7, 85. [Google Scholar] [CrossRef] [PubMed]
- Chandrasekera, P.C.; Pippin, J.J. Of rodents and men: Species-specific glucose regulation and type 2 diabetes research. ALTEX 2014, 31, 157–176. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Lv, M.; Zhu, X.; Wang, H.; Wang, F.; Guan, W. Roles of caloric restriction, ketogenic diet and intermittent fasting during initiation, progression and metastasis of cancer in animal models: A systematic review and meta-analysis. PLoS ONE. 2014, 9, e115147. [Google Scholar] [CrossRef] [PubMed]
- Barañano, K.W.; Hartman, A.L. The ketogenic diet: Uses in epilepsy and other neurologic illnesses. Curr. Treat. Options Neurol. 2008, 10, 410. [Google Scholar] [CrossRef] [PubMed]
- Gouder, N.; Fritschy, J.M.; Boison, D. Seizure suppression by adenosine A1 receptor activation in a mouse model of pharmacoresistant epilepsy. Epilepsia 2003, 44, 877–885. [Google Scholar] [CrossRef] [PubMed]
- Lutas, A.; Yellen, G. The ketogenic diet: Metabolic influences on brain excitability and epilepsy. Trends Neurosci. 2013, 36, 32–40. [Google Scholar] [CrossRef] [PubMed]
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Huang, Q.; Ma, S.; Tominaga, T.; Suzuki, K.; Liu, C. An 8-Week, Low Carbohydrate, High Fat, Ketogenic Diet Enhanced Exhaustive Exercise Capacity in Mice Part 2: Effect on Fatigue Recovery, Post-Exercise Biomarkers and Anti-Oxidation Capacity. Nutrients 2018, 10, 1339. https://doi.org/10.3390/nu10101339
Huang Q, Ma S, Tominaga T, Suzuki K, Liu C. An 8-Week, Low Carbohydrate, High Fat, Ketogenic Diet Enhanced Exhaustive Exercise Capacity in Mice Part 2: Effect on Fatigue Recovery, Post-Exercise Biomarkers and Anti-Oxidation Capacity. Nutrients. 2018; 10(10):1339. https://doi.org/10.3390/nu10101339
Chicago/Turabian StyleHuang, Qingyi, Sihui Ma, Takaki Tominaga, Katsuhiko Suzuki, and Chunhong Liu. 2018. "An 8-Week, Low Carbohydrate, High Fat, Ketogenic Diet Enhanced Exhaustive Exercise Capacity in Mice Part 2: Effect on Fatigue Recovery, Post-Exercise Biomarkers and Anti-Oxidation Capacity" Nutrients 10, no. 10: 1339. https://doi.org/10.3390/nu10101339