3.1. Descriptive Statistics of Soil Properties
A total of 23 soil properties were analyzed as potential soil quality indicators.
Table 2 exhibits minima, maxima, means, standard deviations, and coefficient of variations (CV) for these properties.
Physical soil properties mainly refer to texture, structure, and drainage, which determine the ventilation conditions and thermal properties of soils. These physical properties play an important role in the migration process of water and chemicals in soils. As shown in
Table 2, most soils sampled in this study were classified as sandy loam according to the international system of soil texture classification. The mean proportion of sand, silt and clay in soils from the study area were 71.92%, 18.42%, and 9.32%, respectively. The main issues limiting crop production in these soils are water and nutrient deficiency, and these can be solved via clay application to increase water use efficiency and water conservation [
45]. The bulk density of the soils ranged from 1.11 to 1.59 g cm
−3, with an average value of 1.34 g cm
−3. These values are appropriate for crop root growth in the study area. Studies have shown that root growth was limited when bulk density exceeded 1.5 g cm
−3 due to the high penetration resistance. However, a bulk density less than 1.4 g cm
−3 could effectively alleviate the inhibition of root growth, improve moisture absorption, and increase crop yields [
46]. Soil microaggregates, widely accepted as an indicator of soil structure stability, varied between 58.79% and 95.45% with a mean value of 85.77%. Total porosity as determined by the bulk density, ranged from 0.4 to 0.581 m
3 m
−3 with an average value of 0.49 m
3 m
−3. The minimum value (0.119 m
3 m
−3) of effective porosity was found in fluvo-aquic soils at a height of 3710 m a.s.l. The maximum value (0.274 m
3 m
−3) was obtained from steppe soils at an elevation of 4275 m a.s.l. Water content at field capacity ranged from 0.188 to 0.425 m
3 m
−3, while water content at the permanent wilting point varied between 0.069 and 0.287 m
3 m
−3. Among the 93 sampling points in the study area, 10 had low available water capacity at less than 0.1 m
3 m
−3.
Chemical soil properties related to soil fertility and nutrient management that were analyzed in this study include pH, C, N, P, K, and CEC.
Table 2 shows that there was a fair amount of variation in the soil pH. The soil pH ranged from 4.5 to 8.65, with a mean value of 7.15. The results revealed that only 18 samples from grain agricultural soils exhibited moderately alkaline pH levels. However, almost a third of total samples exhibited acidic pH (<6.5) levels and six samples even exhibited a moderately acidic pH. The acidification of cropland soils in Lhasa Chengguanqu District may be attributed to fertilizer application in the greenhouse systems that grow vegetables [
47]. Total organic C varied between 9.77 and 56.08 g kg
−1, with an average value of 25.44 g kg
−1. Approximately 25.81% of total samples exhibited a total organic C that exceeded 30 g kg
−1, indicating good soil fertility that can support crop growth. The total organic C was significantly correlated with the height above sea level in the study area, with a correlation coefficient of 0.3 (
). A similar relationship was found by Zhang et al. [
48] who reported an increase in total organic C with a rise in the height above sea level. This trend was likely due to the use of animal-derived organic fertilizer in some higher regions. Total N was high across the study area, where 82.80% of soil samples had values more than 1.2 g kg
−1. Among the 93 soil samples analyzed for available N, almost 37% of the samples exhibited high values (>150 mg kg
−1), 53% exhibited medium values (90–150 mg kg
−1), and 10% exhibited low values (<90 mg kg
−1). Total P also exhibited high levels. Total P ranged from 0.44 to 2.88 g kg
−1, with a mean value of 0.89 g kg
−1. Sixty-two sampling points exhibited available P values that exceeded 20 mg kg
−1, while only 5 points had values less than 10 mg kg
−1. The entire study area exhibited medium (15–25 g kg
−1) to high (>25 g kg
−1) levels of total K, and available K exhibited basically similar characteristics. The higher levels of soil nutrients could be attributed to a significant increase in use of nitrogen, phosphorus, and potassium fertilizer that accompanied the comprehensive agricultural development in southern valley area of Tibet that began in 1990. Cation exchange capacity varied between 3.89 and 19.89 cmol kg
−1 of soil, with an average value of 10.74 cmol kg
−1. This indicates a medium capacity for nutrient retention [
49].
For the nutrient cycling process assessment, total organic C was considered as the primary organic matter indicator, with soil enzymes including urease, catalase, alkaline phosphatase, and β-glucosidase. Urease activity ranged from 0.11 to 1.36 mg g
−1 with a mean value of 0.56 mg g
−1. Catalase activity varied between 15.01 and 59.25 mg g
−1, with an average value of 39.00 mg g
−1. Alkaline phosphatase activity varied between 1.36 and 47.28 mg g
−1, with a mean of 17.75 mg g
−1. β-glucosidase activity ranged from 0.27 to 2.71 mg g
−1, with an average value of 1.70 mg g
−1. The average values were similar to those found in previous reports [
50], and are typical for the Lhasa River valley.
Among the 23 soil properties analyzed in this study, bulk density, microaggregates, and total porosity exhibited the least dispersion, with CVs less than 9%. The most variable property was available P, with a CV of 111.61%. The properties with moderate dispersion were silt content, clay content, total P, available K, urease, and alkaline phosphatase. These factors with moderate dispersion exhibited CVs ranging from 40% to 60%.
3.2. Minimum Data Set for Determining Soil Quality
First, the K-S test for goodness of fit was performed to check the normality of soil property data. We used a parametric method if data passed this test, and we used a nonparametric method if data failed the test.
Table 2 shows the parameters of the data distributions and the significance levels of the K-S test. It is obvious that a subset of soil properties, such as bulk density, pH, total C, and catalase, passed the K-S test for normality at a significance level 0.05. However, the data distribution of other soil properties including microaggregates, effective porosity, field capacity, permanent wilting point, total P, available P, available K, and urease were skewed positively or negatively and exhibited leptokurtosis.
Furthermore, comparisons were drawn among groups divided by soil type and agricultural land use for each soil property from the total sampling points as described in
Section 2.3 and statistically significant differences were also identified. For the soil properties with normal distributions, the results of one-way ANOVA revealed that pH, total organic C, total N, available N, CEC, catalase, alkaline phosphatase, and β-glucosidase had significant differences between the groups. There were no significant differences between these groups for any of the physical properties with normal distribution. For the data with non-normal distributions, the results of Kruskal-Wallis test showed that there were significant differences between agricultural land uses and soil types for several physicochemical properties including effective porosity, total P, available P, and available K. As a result, a data set that included the soil properties that exhibited significant differences between the groups was created in preparation for the factor analysis.
The value of the Kaiser-Meyer-Olkin measure of sampling adequacy and Bartlett’s test of sphericity for this set of soil properties are 0.749 and 857.7 respectively [
37]. This implies that the degree of common variance among these indicators was moderate. Therefore, we concluded that the correlations in the soil property data are appropriate for factor analysis. The PCA method with varimax rotation was performed and three principal components were extracted with eigenvalues >1. Most of the common variance shared by the twelve soil indicators was explained by the three principal components, with the cumulative percentage accounting for 68.81% of the variance in the data (
Table 3). Soil indicators associated with each principal component were defined and these indicators exhibited low contributions to other principal components. The first PC, which explained 31.323% of the total variance in the data, was related to pH, available P, catalase, and alkaline phosphatase. The pH, catalase, and alkaline phosphatase are positively loaded on the first PC, while only available P exhibited a negative loading. These indicators revealed that the first PC comprehensively reflected the biochemical status of soils. The second PC explained 28.608% of the total variance and variables that were positively loaded on this factor were total organic C, total N, and available N. Obviously, this set of properties represented soil nutrient status because the variables were mainly related to the nutrients necessary for plants growth. The third PC explained 8.879% of the total variance and effective porosity was positively loaded on this factor. The third PC was principally associated with soil moisture status.
Finally, Pearson’s correlation coefficients were calculated with Equation (2) in
Section 2.4. for the soil indicators in
Table 4. Results show that available P is not significantly correlated with other highly weighted soil indicators in PC1. This lack of collinearity and the high absolute value of factor loading in PC1 indicate that available P is strongly represented in PC1 and should be included in the MDS. In view of the large variance explained by PC1, pH and catalase were also included in MDS to represent the PC1 due to their lower correlation coefficients and high absolute value of factor loading. Because all three highly weighted indicators in PC2 were highly correlated with each other, total organic C and total N were the only variables included in the MDS due to their higher absolute value of factor loading and importance to soil fertility. Effective porosity was directly retained in the MDS because it was the only highly weighted indicator in the PC3. Consequently, the final MDS for determining soil quality in the study area comprised six indicators: effective porosity, pH, total organic C, total N, available P, and catalase.
Almost all the soil properties have been identified in previous studies as important indicators for soil quality evaluation under different agricultural land uses. Shukla et al. [
14] reported effective porosity as an important indicator for assessing soil quality in the surface layer of soil under different land uses and management practices. Effective porosity has also been mentioned as one of the major determining factors that controls yield variability [
51], and it has been suggested as an indicator for routine evaluation and monitoring of soil physical quality [
52]. pH was identified as a soil quality indicator by Andrews et al. [
35], Shukla et al. [
14], and Bautista-Cruz et al. [
41]. Meng et al. [
30] also suggested that pH is an important evaluation indicator due to its significant impact on the availability and form of soil nutrients. However, pH was the first factor eliminated in the study of Rezaei et al. [
13] because its range of values within the study area was insufficient to result in substantial differences in plant growth. Soil properties related to organic matter or organic carbon were regarded as the most dominant indicator for soil quality [
36,
41,
53,
54]. These factors can be monitored over time to determine if soil quality has improved, degraded, or remained stable [
14]. Pan et al. [
55] reported that total organic C plays a crucial role in sequestration of C, enhancement of crop productivity, and stabilization of yield in China’s croplands. In the Tibetan Plateau, higher total organic C in cropland soils usually implies superior soil quality. Total N has been reported as an important soil quality indicator for different management system treatments such as crop rotation and use of external inputs [
35]. Total N at a soil depth of 10–20 cm is more important for assessing soil quality in comparison with total N at soil depths in the range of 0–10 cm [
14]. However, total N was reported as a key soil quality indicator to represent soil fertility at the 0–20 cm soil depth for coastal tidal lands [
30]. Liu et al. [
56] also reported that total N was a major determinant and indicator of soil fertility and quality in agricultural ecosystems. Available P was identified as one of the most important indicators for assessing soil quality within an agricultural watershed [
15]. Chen et al. [
57] reported that available P was selected as an important soil quality indicator and had considerable effect on soybean yield. Available P was regarded as the primary limiting factor for rice growth in the red soil region, and it was included in the MDS of soil quality created by Li et al. [
36] and Meng et al. [
30]. Catalase exhibited a strong positive correlation with the content of soil nutrients, and it can be regarded as an important soil quality indicator in the desertification process [
58]. Similar results were reported by Zhang et al. [
59], and catalase was selected in previous study that calculated a soil quality index in the Loess Plateau [
60].
3.3. Soil Quality Index under Different Soil Types and Land Use Types
The soil indicators in the final MDS were transformed via the membership function as described in
Section 2.4 with Equations (4) and (5). The critical values of the soil quality indicators were determined according to the ecological requirements of local crops in the study area in conjunction with the previous research results (
Table 5). Effective porosity is indispensable to ensure soil air quality for normal growth of plants and microorganism activities, and a upper half trapezoid curve function was therefore used to calculate scores for the indicator [
14]. The optimum pH range for most crops in Tibet is between 6.9 and 7.1 [
61], and this range of pH values received a score of 1. Scores for pH values <6.9 or >7.1 were determined by a trapezoid curve function [
7]. The scores for total organic C were determined by the upper half trapezoid curve function based on the critical role for soil fertility [
7,
62,
63]. For total N and available P, scores were assigned by the upper half trapezoid curve function because these are the nutrients that most frequently limit crop productivity [
62,
64]. Catalase activity reflects the intensity of soil microbial activity in the study area which has a positive effect on soil quality. Therefore, the upper half trapezoid curve function was used for scoring [
65].
The soil quality index (SQI) was obtained via a linear weighted sum of the scores of all six indicators for each of the 93 sampling points as described in
Section 2.4 with Equation (6). The average SQI value was 0.603, while the minimum and maximum was 0.350 and 0.816, respectively, for points situated in grain land with fluvo-aquic soils and meadow soils. The SQI results in
Figure 2 show the following order of the groups according to soil types and land use types: grain land with fluvo-aquic soils < conventional vegetable land with fluvo-aquic soils < greenhouse vegetable land with fluvo-aquic soils < grain land with steppe soils < grain land with meadow soils. Soil quality indices were affected by the coupling of soil types and land use types in the study area.
The SQI values were significantly higher in grain land with meadow soils with an average value 0.711. The SQI of other groups ranged from 0.545 in grain land with fluvo-aquic soils to 0.625 in grain land with steppe soils. SQI values less than 0.5 were almost all located in grain land with fluvo-aquic soils. Conventional vegetable land with fluvo-aquic soils exhibited moderate SQI values. The difference in SQI indices between these groups can be attributed to different soil characteristics, tillage practices, and fertilizer application.
Both the meadow soils and steppe soils developed from calcareous parent material originating from alluvial deposits. These soils were characterized by a thick tillage layer, fine soil structure, and high soil nutrient content. These soils were highly suitable for the growth of barley, wheat, peas, and rapeseed. Meadow soils had a higher capability to retain water and nutrients, while N and P content in steppe soils were slightly insufficient [
61]. Although fluvo-aquic soils were located in valley terraces with superior irrigation conditions, these soils formed in noncalcareous parent material from alluvial deposits and exhibited high sand contents. This results in a low ability to retain nutrients or water. Furthermore, these soils exhibited lower values of nutrient content and a notable lack of available nutrients. It is obvious that the soil quality with meadow soils was superior to steppe soils and fluvo-aquic soils given that these soils were not cultivated.
When considering land use types, 68% sampling points of fluvo-aquic soils, all of meadow soils, and all of steppe soils were from the grain land areas, while 11% of fluvo-aquic soils were from the conventional vegetable land areas and 21% of fluvo-aquic soils were from the greenhouse vegetable land areas, respectively. In
Table 6, all the soil quality indices except catalase for fluvo-aquic soils were significantly lower in the grain land areas when compared with the conventional vegetable land areas and the greenhouse vegetable land areas. Compared to the soils from grain land, soils from vegetable land exhibited lower values of effective porosity, pH and catalase as well as higher values of total organic C, total N and available P (
Table 6). Oliveira et al. [
66] found that conventional tillage with mineral fertilizer and pig slurry application increased the bulk density and penetration resistance, resulting in the reduction of effective porosity. Reynolds et al. [
67] observed that effective porosity was critically low under a system of long-term (48 years) cropping, fertilization and fall moldboard plow tillage. The main reason for the low pH value in vegetable land may be due to the excessive application of N fertilizer. It has been observed that average soil pH values declined significantly over time as N application rates have slightly increased [
68]. Lower catalase activities were found in vegetable land. This is attributed to the application of acid fertilizer and unleavened manure, resulting in enhancements of harmful bacteria and element activity [
69]. The total organic C, total N and available P were significantly higher in vegetable land This was caused by the application of N and phosphorus fertilizer and organic fertilizer. Soil organic C accumulates gradually with long-term cultivation and fertilizer application [
70]. Significantly higher accumulation of available N and P were observed under application of inorganic fertilizers and manure either alone or in combination [
71].