Pharmaceutics 2014, 6(1), 185-194; doi:10.3390/pharmaceutics6010185

Encapsulation of Alcohol Dehydrogenase in Mannitol by Spray Drying

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Received: 17 January 2014; in revised form: 27 February 2014 / Accepted: 13 March 2014 / Published: 24 March 2014
(This article belongs to the Special Issue Microencapsulation Technology Applied to Pharmaceutics 2014)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The retention of the enzyme activity of alcohol dehydrogenase (ADH) has been studied in various drying processes such as spray drying. The aim of this study is to encapsulate ADH in mannitol, either with or without additive in order to limit the thermal denaturation of the enzyme during the drying process. The retention of ADH activity was investigated at different drying temperatures. When mannitol was used, the encapsulated ADH was found inactive in all the dried powders. This is presumably due to the quick crystallization of mannitol during spray drying that resulted in the impairment of enzyme protection ability in comparison to its amorphous form. Maltodextin (dextrose equivalent = 11) was used to reduce the crystallization of mannitol. The addition of maltodextrin increased ADH activity and drastically changed the powder X-ray diffractogram of the spray-dried powders.
Keywords: alcohol dehydrogenase; encapsulation; spray drying; mannitol
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MDPI and ACS Style

Shiga, H.; Joreau, H.; Neoh, T.L.; Furuta, T.; Yoshii, H. Encapsulation of Alcohol Dehydrogenase in Mannitol by Spray Drying. Pharmaceutics 2014, 6, 185-194.

AMA Style

Shiga H, Joreau H, Neoh TL, Furuta T, Yoshii H. Encapsulation of Alcohol Dehydrogenase in Mannitol by Spray Drying. Pharmaceutics. 2014; 6(1):185-194.

Chicago/Turabian Style

Shiga, Hirokazu; Joreau, Hiromi; Neoh, Tze L.; Furuta, Takeshi; Yoshii, Hidefumi. 2014. "Encapsulation of Alcohol Dehydrogenase in Mannitol by Spray Drying." Pharmaceutics 6, no. 1: 185-194.

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