Molecular Mechanisms of Inhibitory Effects of Bovine Lactoferrin on Invasion of Oral Squamous Cell Carcinoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cells and Cell Culture
2.3. Wound Healing Assay
2.4. Chamber Migration Assay
2.5. In Vitro Invasion Assay
2.6. Reverse Transcription Polymerase Chain Reaction (RT-PCR)
2.7. Quantitative RT-PCR (qRT-PCR)
2.8. Western Blotting Analysis
2.9. Silencing by Small Interfering RNA (SiRNA)
2.10. Statistical Analysis
3. Results
3.1. Bovine Lactoferrin Inhibits Invasion of OSCC Cells
3.2. Bovine Lactoferrin Suppresses Invasion of OSCC Cells by Controlling AP-1 Activity to Suppress Expression of MMP-1 and MMP-3
3.3. Bovine Lactoferrin Requires Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) to Inhibit OSCC Cell Invasion
3.4. Bovine Lactoferrin Suppresses tPA-Induced Cell Invasion of OSCC by Reducing MMP-1 and MMP-3 Production
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chea, C.; Miyauchi, M.; Inubushi, T.; Okamoto, K.; Haing, S.; Takata, T. Molecular Mechanisms of Inhibitory Effects of Bovine Lactoferrin on Invasion of Oral Squamous Cell Carcinoma. Pharmaceutics 2023, 15, 562. https://doi.org/10.3390/pharmaceutics15020562
Chea C, Miyauchi M, Inubushi T, Okamoto K, Haing S, Takata T. Molecular Mechanisms of Inhibitory Effects of Bovine Lactoferrin on Invasion of Oral Squamous Cell Carcinoma. Pharmaceutics. 2023; 15(2):562. https://doi.org/10.3390/pharmaceutics15020562
Chicago/Turabian StyleChea, Chanbora, Mutsumi Miyauchi, Toshihiro Inubushi, Kana Okamoto, Sivmeng Haing, and Takashi Takata. 2023. "Molecular Mechanisms of Inhibitory Effects of Bovine Lactoferrin on Invasion of Oral Squamous Cell Carcinoma" Pharmaceutics 15, no. 2: 562. https://doi.org/10.3390/pharmaceutics15020562