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Viruses 2017, 9(7), 183; doi:10.3390/v9070183

Variability of Emaravirus Species Associated with Sterility Mosaic Disease of Pigeonpea in India Provides Evidence of Segment Reassortment

ICAR-National Research Centre on Plant Biotechnology, IARI, Pusa Campus, New Delhi 110012, India
Author to whom correspondence should be addressed.
Received: 16 May 2017 / Revised: 4 July 2017 / Accepted: 6 July 2017 / Published: 11 July 2017
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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Sterility mosaic disease (SMD) of pigeonpea is a serious constraint for cultivation of pigeonpea in India and other South Asian countries. SMD of pigeonpea is associated with two distinct emaraviruses, Pigeonpea sterility mosaic virus 1 (PPSMV-1) and Pigeonpea sterility mosaic virus 2 (PPSMV-2), with genomes consisting of five and six negative-sense RNA segments, respectively. The recently published genome sequences of both PPSMV-1 and PPSMV-2 are from a single location, Patancheru from the state of Telangana in India. However, here we present the first report of sequence variability among 23 isolates of PPSMV-1 and PPSMV-2, collected from ten locations representing six states of India. Both PPSMV-1 and PPSMV-2 are shown to be present across India and to exhibit considerable sequence variability. Variability of RNA3 sequences was higher than the RNA4 sequences for both PPSMV-1 and PPSMV-2. Additionally, the sixth RNA segment (RNA6), previously reported to be associated with only PPSMV-2, is also associated with isolates of PPSMV-1. Multiplex reverse transcription PCR (RT-PCR) analyses show that PPSMV-1 and PPSMV-2 frequently occur as mixed infections. Further sequence analyses indicated the presence of reassortment of RNA4 between isolates of PPSMV-1 and PPSMV-2. View Full-Text
Keywords: pigeonpea sterility mosaic virus; emaravirus; variability; reassortment pigeonpea sterility mosaic virus; emaravirus; variability; reassortment

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Patil, B.L.; Dangwal, M.; Mishra, R. Variability of Emaravirus Species Associated with Sterility Mosaic Disease of Pigeonpea in India Provides Evidence of Segment Reassortment. Viruses 2017, 9, 183.

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