Next Article in Journal
Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
Previous Article in Journal
Effective Suckling C57BL/6, Kunming, and BALB/c Mouse Models with Remarkable Neurological Manifestation for Zika Virus Infection
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Viruses 2017, 9(7), 166; doi:10.3390/v9070166

Systemic Propagation of a Fluorescent Infectious Clone of a Polerovirus Following Inoculation by Agrobacteria and Aphids

1
Université de Strasbourg, Institut National de la Recherche Agronomique, SVQV UMR-A 1131, 68000 Colmar, France
2
UMR 385 BGPI, Institut National de la Recherche Agronomique—Centre de Coopération Internationale en Recherche Agronomique pour le Développement, SupAgro, CIRAD TA-A54/K, Campus International de Baillarguet, 34398 Montpellier, France
3
Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, UPR 2357, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France
4
Institute of Plant Science Paris Saclay (IPS2), CNRS, INRA, University Paris Diderot, University of Paris-Saclay, 91405 Orsay, France
*
Author to whom correspondence should be addressed.
Academic Editor: K. Andrew White
Received: 3 May 2017 / Revised: 16 June 2017 / Accepted: 22 June 2017 / Published: 29 June 2017
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
View Full-Text   |   Download PDF [2781 KB, uploaded 29 June 2017]   |  

Abstract

A fluorescent viral clone of the polerovirus Turnip yellows virus (TuYV) was engineered by introducing the Enhanced Green Fluorescent Protein (EGFP) sequence into the non-structural domain sequence of the readthrough protein, a minor capsid protein. The resulting recombinant virus, referred to as TuYV-RTGFP, was infectious in several plant species when delivered by agroinoculation and invaded efficiently non-inoculated leaves. As expected for poleroviruses, which infect only phloem cells, the fluorescence emitted by TuYV-RTGFP was restricted to the vasculature of infected plants. In addition, TuYV-RTGFP was aphid transmissible and enabled the observation of the initial sites of infection in the phloem after aphid probing in epidermal cells. The aphid-transmitted virus moved efficiently to leaves distant from the inoculation sites and importantly retained the EGFP sequence in the viral genome. This work reports on the first engineered member in the Luteoviridae family that can be visualized by fluorescence emission in systemic leaves of different plant species after agroinoculation or aphid transmission. View Full-Text
Keywords: polerovirus; fluorescent clone; systemic infection; aphid transmission polerovirus; fluorescent clone; systemic infection; aphid transmission
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Boissinot, S.; Pichon, E.; Sorin, C.; Piccini, C.; Scheidecker, D.; Ziegler-Graff, V.; Brault, V. Systemic Propagation of a Fluorescent Infectious Clone of a Polerovirus Following Inoculation by Agrobacteria and Aphids. Viruses 2017, 9, 166.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top