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Viruses 2017, 9(12), 379; doi:10.3390/v9120379

A Two-Dimensional Human Minilung System (Model) for Respiratory Syncytial Virus Infections

1
Functional Unit for Research into Chronic Diseases (UFIEC), Institute of Health Carlos III, 28220 Madrid, Spain
2
Spanish National Center for Microbiology (CNM), Institute of Health Carlos III, 28220 Madrid, Spain
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 9 November 2017 / Revised: 4 December 2017 / Accepted: 8 December 2017 / Published: 10 December 2017
(This article belongs to the Section Animal Viruses)
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Abstract

Human respiratory syncytial virus (HRSV) is a major cause of serious pediatric respiratory diseases that lacks effective vaccine or specific therapeutics. Although our understanding about HRSV biology has dramatically increased during the last decades, the need for adequate models of HRSV infection is compelling. We have generated a two-dimensional minilung from human embryonic stem cells (hESCs). The differentiation protocol yielded at least six types of lung and airway cells, although it is biased toward the generation of distal cells. We show evidence of HRSV replication in lung cells, and the induction of innate and proinflammatory responses, thus supporting its use as a model for the study of HRSV–host interactions. View Full-Text
Keywords: human embryonic stem cells; epithelial cells; respiratory syncytial virus; minilung human embryonic stem cells; epithelial cells; respiratory syncytial virus; minilung
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Magro-Lopez, E.; Guijarro, T.; Martinez, I.; Martin-Vicente, M.; Liste, I.; Zambrano, A. A Two-Dimensional Human Minilung System (Model) for Respiratory Syncytial Virus Infections. Viruses 2017, 9, 379.

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