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Viruses 2016, 8(5), 126; doi:10.3390/v8050126

Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus Infection

1
Laboratory of Cell Signal Transduction, Medical School, Henan University, Kaifeng 475004, China
2
School of Education Science, Henan University, Kaifeng 475004, China
3
Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Karyn Johnson
Received: 16 February 2016 / Revised: 20 April 2016 / Accepted: 26 April 2016 / Published: 7 May 2016
(This article belongs to the Section Insect Viruses)
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Abstract

At present, the details of lamina alterations after baculovirus infection remain elusive. In this study, a lamin gene in the Sf9 cell line of Spodoptera frugiperda was cloned. The open reading frame (orf) of the Sf9 lamin was 1860 bp and encoded a protein with a molecular weight of 70 kDa. A transfection assay with a red fluorescence protein (rfp)-lamin fusion protein indicated that Sf9 lamin was localized in the nuclear rim. Transmission electron microscopy observations indicated that Autographa californica multiple nucleopolyhedrovirus (AcMNPV) nucleocapsids may pass through the nuclear envelope. Immunofluorescence assay indicated that the lamina showed a ruffled staining pattern with the formation of invaginations in the Sf9 cells infected with AcMNPV, while it was evenly distributed at the nuclear periphery of mock-infected cells. Western blotting results indicated that the total amount of lamin in the baculovirus-infected Sf9 cells was significantly decreased compared with the mock-infected cells. These results imply that AcMNPV infection induces structural and biochemical rearrangements of lamina of Sf9 cells. View Full-Text
Keywords: Autographa californica multiple nucleopolyhedrovirus; infection; Sf9 cells; lamina Autographa californica multiple nucleopolyhedrovirus; infection; Sf9 cells; lamina
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wei, W.; Wang, H.; Li, X.; Fang, N.; Yang, S.; Liu, H.; Kang, X.; Sun, X.; Ji, S. Cloning and Characterization of Sf9 Cell Lamin and the Lamin Conformational Changes during Autographa californica multiple nucleopolyhedrovirus Infection. Viruses 2016, 8, 126.

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