Next Article in Journal
Characterization of Intra-Type Variants of Oncogenic Human Papillomaviruses by Next-Generation Deep Sequencing of the E6/E7 Region
Previous Article in Journal
Characterization of Viral Communities of Biting Midges and Identification of Novel Thogotovirus Species and Rhabdovirus Genus
Article Menu

Export Article

Open AccessArticle
Viruses 2016, 8(3), 65; doi:10.3390/v8030065

Isolation of a Novel Fusogenic Orthoreovirus from Eucampsipoda africana Bat Flies in South Africa

1
Centre for Emerging and Zoonotic Diseases, National Institute for Communicable Diseases, National Health Laboratory Service, Sandringham 2131, South Africa
2
Department of Microbiology and Plant Pathology, Faculty of Natural and Agricultural Science, University of Pretoria, Pretoria 0028, South Africa
3
Center for Genomic Science, United States Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA
4
Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2193, South Africa
*
Author to whom correspondence should be addressed.
Academic Editor: Andrew Mehle
Received: 27 November 2015 / Revised: 26 January 2016 / Accepted: 23 February 2016 / Published: 29 February 2016
(This article belongs to the Section Animal Viruses)
View Full-Text   |   Download PDF [5617 KB, uploaded 14 March 2016]   |  

Abstract

We report on the isolation of a novel fusogenic orthoreovirus from bat flies (Eucampsipoda africana) associated with Egyptian fruit bats (Rousettus aegyptiacus) collected in South Africa. Complete sequences of the ten dsRNA genome segments of the virus, tentatively named Mahlapitsi virus (MAHLV), were determined. Phylogenetic analysis places this virus into a distinct clade with Baboon orthoreovirus, Bush viper reovirus and the bat-associated Broome virus. All genome segments of MAHLV contain a 5' terminal sequence (5'-GGUCA) that is unique to all currently described viruses of the genus. The smallest genome segment is bicistronic encoding for a 14 kDa protein similar to p14 membrane fusion protein of Bush viper reovirus and an 18 kDa protein similar to p16 non-structural protein of Baboon orthoreovirus. This is the first report on isolation of an orthoreovirus from an arthropod host associated with bats, and phylogenetic and sequence data suggests that MAHLV constitutes a new species within the Orthoreovirus genus. View Full-Text
Keywords: Rousettus aegyptiacus; fusogenic orthoreovirus; Reoviridae; Nycteribiidae; bat flies; Eucampsipoda africana Rousettus aegyptiacus; fusogenic orthoreovirus; Reoviridae; Nycteribiidae; bat flies; Eucampsipoda africana
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Jansen van Vuren, P.; Wiley, M.; Palacios, G.; Storm, N.; McCulloch, S.; Markotter, W.; Birkhead, M.; Kemp, A.; Paweska, J.T. Isolation of a Novel Fusogenic Orthoreovirus from Eucampsipoda africana Bat Flies in South Africa. Viruses 2016, 8, 65.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top