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Viruses 2016, 8(3), 59; doi:10.3390/v8030059

Monitoring Physiological Changes in Haloarchaeal Cell during Virus Release

1
Department of Biosciences, Institute of Biotechnology, University of Helsinki, Viikinkaari 9, 00014 Helsinki, Finland
2
Department of Biochemistry, Vytautas Magnus University, Vileikos g. 8, 44404 Kaunas, Lithuania
*
Author to whom correspondence should be addressed.
Academic Editor: Rob Lavigne
Received: 7 December 2015 / Revised: 11 February 2016 / Accepted: 15 February 2016 / Published: 24 February 2016
(This article belongs to the Section Bacterial Viruses)
View Full-Text   |   Download PDF [2331 KB, uploaded 24 February 2016]   |  

Abstract

The slow rate of adsorption and non-synchronous release of some archaeal viruses have hindered more thorough analyses of the mechanisms of archaeal virus release. To address this deficit, we utilized four viruses that infect Haloarcula hispanica that represent the four virion morphotypes currently known for halophilic euryarchaeal viruses: (1) icosahedral internal membrane-containing SH1; (2) icosahedral tailed HHTV-1; (3) spindle-shaped His1; and (4) pleomorphic His2. To discern the events occurring as the progeny viruses exit, we monitored culture turbidity, as well as viable cell and progeny virus counts of infected and uninfected cultures. In addition to these traditional metrics, we measured three parameters associated with membrane integrity: the binding of the lipophilic anion phenyldicarbaundecaborane, oxygen consumption, and both intra- and extra-cellular ATP levels. View Full-Text
Keywords: Haloarcula hispanica; icosahedral membrane-containing virus SH1; icosahedral tailed virus HHTV-1; spindle-shaped virus His1; pleomorphic virus His2; potentiometry; virus life cycle; Pleolipoviridae; Sphaerolipoviridae Haloarcula hispanica; icosahedral membrane-containing virus SH1; icosahedral tailed virus HHTV-1; spindle-shaped virus His1; pleomorphic virus His2; potentiometry; virus life cycle; Pleolipoviridae; Sphaerolipoviridae
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Svirskaitė, J.; Oksanen, H.M.; Daugelavičius, R.; Bamford, D.H. Monitoring Physiological Changes in Haloarchaeal Cell during Virus Release. Viruses 2016, 8, 59.

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