Next Article in Journal
Pharmacokinetics of the Antiviral Lectin Griffithsin Administered by Different Routes Indicates Multiple Potential Uses
Previous Article in Journal
RNA Interference in Insect Vectors for Plant Viruses
Article Menu

Export Article

Open AccessArticle
Viruses 2016, 8(12), 330; doi:10.3390/v8120330

Genetic Structure and Molecular Variability Analysis of Citrus sudden death-associated virus Isolates from Infected Plants Grown in Brazil

1
Instituto de Biociências de Botucatu, Universidade Estadual Paulista, Botucatu, São Paulo 03178-200, Brazil
2
Laboratório de Biotecnologia, Centro de Citricultura Sylvio Moreira, Instituto Agronômico, Cordeiropolis, SP 13490-970, Brazil
3
Department of Plant Pathology, University of California Davis, Davis, CA 95616, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas Hohn
Received: 13 October 2016 / Revised: 9 December 2016 / Accepted: 10 December 2016 / Published: 16 December 2016
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
View Full-Text   |   Download PDF [8364 KB, uploaded 16 December 2016]   |  

Abstract

Citrus sudden death-associated virus (CSDaV) is a monopartite positive-sense single-stranded RNA virus that was suggested to be associated with citrus sudden death (CSD) disease in Brazil. Here, we report the first study of the genetic structure and molecular variability among 31 CSDaV isolates collected from both symptomatic and asymptomatic trees in CSD-affected areas. Analyses of partial nucleotide sequences of five domains of the CSDaV genomic RNA, including those encoding for the methyltransferase, the multi-domain region (MDR), the helicase, the RNA-dependent RNA polymerase and the coat protein, showed that the MDR coding region was the most diverse region assessed here, and a possible association between this region and virus adaption to different host or plant tissues is considered. Overall, the nucleotide diversity (π) was low for CSDaV isolates, but the phylogenetic analyses revealed the predominance of two main groups, one of which showed a higher association with CSD-symptomatic plants. Isolates obtained from CSD-symptomatic plants, compared to those obtained from asymptomatic plants, showed higher nucleotide diversity, nonsynonymous and synonymous substitution rates and number of amino acid changes on the coding regions located closer to the 5’ end region of the genomic RNA. This work provides new insights into the genetic diversity of the CSDaV, giving support for further epidemiological studies. View Full-Text
Keywords: citrus sudden death; CSDaV; plant virus; Marafivirus; diversity citrus sudden death; CSDaV; plant virus; Marafivirus; diversity
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Matsumura, E.E.; Coletta Filho, H.D.; de Oliveira Dorta, S.; Nouri, S.; Machado, M.A. Genetic Structure and Molecular Variability Analysis of Citrus sudden death-associated virus Isolates from Infected Plants Grown in Brazil. Viruses 2016, 8, 330.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top