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Viruses 2016, 8(11), 315; doi:10.3390/v8110315

Aphis Glycines Virus 2, a Novel Insect Virus with a Unique Genome Structure

Department of Entomology, Iowa State University, Ames, IA 50011, USA
Present address: Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.
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Author to whom correspondence should be addressed.
Academic Editor: Karyn Johnson
Received: 2 September 2016 / Revised: 27 October 2016 / Accepted: 8 November 2016 / Published: 19 November 2016
(This article belongs to the Section Insect Viruses)
View Full-Text   |   Download PDF [2208 KB, uploaded 19 November 2016]   |  

Abstract

The invasive soybean aphid, Aphis glycines, is a major pest in soybeans, resulting in substantial economic loss. We analyzed the A. glycines transcriptome to identify sequences derived from viruses of A. glycines. We identified sequences derived from a novel virus named Aphis glycines virus 2 (ApGlV2). The assembled virus genome sequence was confirmed by reverse transcription polymerase chain reaction (RT-PCR) and Sanger sequencing, conserved domains were characterized, and distribution, and transmission examined. This virus has a positive sense, single-stranded RNA genome of ~4850 nt that encodes three proteins. The RNA-dependent RNA polymerase (RdRp) of ApGlV2 is a permuted RdRp similar to those of some tetraviruses, while the capsid protein is structurally similar to the capsid proteins of plant sobemoviruses. ApGlV2 also encodes a larger minor capsid protein, which is translated by a readthrough mechanism. ApGlV2 appears to be widespread in A. glycines populations and to persistently infect aphids with a 100% vertical transmission rate. ApGlV2 is susceptible to the antiviral RNA interference (RNAi) pathway. This virus, with its unique genome structure with both plant- and insect-virus characteristics, is of particular interest from an evolutionary standpoint. View Full-Text
Keywords: insect virus; soybean aphid; plant virus; readthrough domain; small RNA virus insect virus; soybean aphid; plant virus; readthrough domain; small RNA virus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Liu, S.; Vijayendran, D., 1; Chen, Y.; Bonning, B.C. Aphis Glycines Virus 2, a Novel Insect Virus with a Unique Genome Structure. Viruses 2016, 8, 315.

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