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Viruses 2016, 8(10), 265; doi:10.3390/v8100265

The VP1u Receptor Restricts Parvovirus B19 Uptake to Permissive Erythroid Cells

1
Department of Chemistry and Biochemistry, University of Bern, Bern 3012, Switzerland
2
CSL Behring AG, Bern 3014, Switzerland
*
Author to whom correspondence should be addressed.
Academic Editor: Joanna Parish
Received: 29 August 2016 / Revised: 19 September 2016 / Accepted: 20 September 2016 / Published: 28 September 2016
(This article belongs to the Section Animal Viruses)
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Abstract

Parvovirus B19 (B19V) is a small non-enveloped virus and known as the causative agent for the mild childhood disease erythema infectiosum. B19V has an extraordinary narrow tissue tropism, showing only productive infection in erythroid precursor cells in the bone marrow. We recently found that the viral protein 1 unique region (VP1u) contains an N-terminal receptor-binding domain (RBD), which mediates the uptake of the virus into cells of the erythroid lineage. To further investigate the role of the RBD in connection with a B19V-unrelated capsid, we chemically coupled the VP1u of B19V to the bacteriophage MS2 capsid and tested the internalization capacity of the bioconjugate on permissive cells. In comparison, we studied the cellular uptake and infection of B19V along the erythroid differentiation. The results showed that the MS2-VP1u bioconjugate mimicked the specific internalization of the native B19V into erythroid precursor cells, which further coincides with the restricted infection profile. The successful mimicry of B19V uptake demonstrates that the RBD in the VP1u is sufficient for the endocytosis of the viral capsid. Furthermore, the recombinant VP1u competed with B19V uptake into permissive cells, thus excluding a significant alternative uptake mechanism by other receptors. Strikingly, the VP1u receptor appeared to be expressed only on erythropoietin-dependent erythroid differentiation stages that also provide the necessary intracellular factors for a productive infection. Taken together, these findings suggest that the VP1u binds to a yet-unknown erythroid-specific cellular receptor and thus restricts the virus entry to permissive cells. View Full-Text
Keywords: parvovirus B19; virus entry; VP1u; VP1u receptor; tropism; erythropoiesis; virus mimicry; MS2 bioconjugation parvovirus B19; virus entry; VP1u; VP1u receptor; tropism; erythropoiesis; virus mimicry; MS2 bioconjugation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Leisi, R.; Von Nordheim, M.; Ros, C.; Kempf, C. The VP1u Receptor Restricts Parvovirus B19 Uptake to Permissive Erythroid Cells. Viruses 2016, 8, 265.

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