Next Article in Journal
HTLV-1, Immune Response and Autoimmunity
Previous Article in Journal
Newcastle Disease Virus: Potential Therapeutic Application for Human and Canine Lymphoma
Article Menu

Export Article

Open AccessArticle
Viruses 2016, 8(1), 2; doi:10.3390/v8010002

TRIM19/PML Restricts HIV Infection in a Cell Type-Dependent Manner

1
Institute of Clinical and Molecular Virology, Friedrich-Alexander University Erlangen-Nürnberg, 91054 Erlangen, Germany
2
Institute of Clinical Chemistry and Pharmacology, University Hospital Bonn, 53127 Bonn, Germany
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Andrew Mehle
Received: 7 August 2015 / Revised: 2 December 2015 / Accepted: 14 December 2015 / Published: 23 December 2015
(This article belongs to the Section Animal Viruses)
View Full-Text   |   Download PDF [4863 KB, uploaded 23 December 2015]   |  

Abstract

The promyelocytic leukemia protein (PML) is the main structural component of the nuclear matrix structures termed nuclear domain 10 (ND10) or PML nuclear bodies (PML-NBs). PML and ND10 structures have been shown to mediate an intrinsic immune response against a variety of different viruses. Their role during retroviral replication, however, is still controversially discussed. In this study, we analyzed the role of PML and the ND10 components Daxx and Sp100 during retroviral replication in different cell types. Using cell lines exhibiting a shRNA-mediated knockdown, we found that PML, but not Daxx or Sp100, inhibits HIV and other retroviruses in a cell type-dependent manner. The PML-mediated block to retroviral infection was active in primary human fibroblasts and murine embryonic fibroblasts but absent from T cells and myeloid cell lines. Quantitative PCR analysis of HIV cDNA in infected cells revealed that PML restricts infection at the level of reverse transcription. Our findings shed light on the controversial role of PML during retroviral infection and show that PML contributes to the intrinsic restriction of retroviral infections in a cell type-dependent manner. View Full-Text
Keywords: PML; TRIM19; ND10; HIV-1; retrovirus; intrinsic immunity PML; TRIM19; ND10; HIV-1; retrovirus; intrinsic immunity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kahle, T.; Volkmann, B.; Eissmann, K.; Herrmann, A.; Schmitt, S.; Wittmann, S.; Merkel, L.; Reuter, N.; Stamminger, T.; Gramberg, T. TRIM19/PML Restricts HIV Infection in a Cell Type-Dependent Manner. Viruses 2016, 8, 2.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top