Next Article in Journal
Bio-Control of Salmonella Enteritidis in Foods Using Bacteriophages
Previous Article in Journal
Cloak and Dagger: Alternative Immune Evasion and Modulation Strategies of Poxviruses
Article Menu

Export Article

Open AccessCommentary
Viruses 2015, 7(8), 4826-4835; doi:10.3390/v7082846

New Structure Sheds Light on Selective HIV-1 Genomic RNA Packaging

Department of Chemistry and Biochemistry, Center for Retrovirus Research, and Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Rachel van Duyne
Received: 27 July 2015 / Revised: 17 August 2015 / Accepted: 18 August 2015 / Published: 24 August 2015
(This article belongs to the Section Editorial)
View Full-Text   |   Download PDF [271 KB, uploaded 24 August 2015]   |  

Abstract

Two copies of unspliced human immunodeficiency virus (HIV)-1 genomic RNA (gRNA) are preferentially selected for packaging by the group-specific antigen (Gag) polyprotein into progeny virions as a dimer during the late stages of the viral lifecycle. Elucidating the RNA features responsible for selective recognition of the full-length gRNA in the presence of an abundance of other cellular RNAs and spliced viral RNAs remains an area of intense research. The recent nuclear magnetic resonance (NMR) structure by Keane et al. [1] expands upon previous efforts to determine the conformation of the HIV-1 RNA packaging signal. The data support a secondary structure wherein sequences that constitute the major splice donor site are sequestered through base pairing, and a tertiary structure that adopts a tandem 3-way junction motif that exposes the dimerization initiation site and unpaired guanosines for specific recognition by Gag. While it remains to be established whether this structure is conserved in the context of larger RNA constructs or in the dimer, this study serves as the basis for characterizing large RNA structures using novel NMR techniques, and as a major advance toward understanding how the HIV-1 gRNA is selectively packaged. View Full-Text
Keywords: HIV; RNA structure; retroviral packaging; NMR; psi HIV; RNA structure; retroviral packaging; NMR; psi
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Olson, E.D.; Cantara, W.A.; Musier-Forsyth, K. New Structure Sheds Light on Selective HIV-1 Genomic RNA Packaging. Viruses 2015, 7, 4826-4835.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top