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Viruses 2015, 7(8), 4563-4581; doi:10.3390/v7082833

eEF1A Interacts with the NS5A Protein and Inhibits the Growth of Classical Swine Fever Virus

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, Heilongjiang, China
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Eric O. Freed
Received: 3 July 2015 / Revised: 3 August 2015 / Accepted: 5 August 2015 / Published: 10 August 2015
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Abstract

The NS5A protein of classical swine fever virus (CSFV) is involved in the RNA synthesis and viral replication. However, the NS5A-interacting cellular proteins engaged in the CSFV replication are poorly defined. Using yeast two-hybrid screen, the eukaryotic elongation factor 1A (eEF1A) was identified to be an NS5A-binding partner. The NS5A–eEF1A interaction was confirmed by coimmunoprecipitation, glutathione S-transferase (GST) pulldown and laser confocal microscopy assays. The domain I of eEF1A was shown to be critical for the NS5A–eEF1A interaction. Overexpression of eEF1A suppressed the CSFV growth markedly, and conversely, knockdown of eEF1A enhanced the CSFV replication significantly. Furthermore, eEF1A, as well as NS5A, was found to reduce the translation efficiency of the internal ribosome entry site (IRES) of CSFV in a dose-dependent manner, as demonstrated by luciferase reporter assay. Streptavidin pulldown assay revealed that eEF1A could bind to the CSFV IRES. Collectively, our results suggest that eEF1A interacts with NS5A and negatively regulates the growth of CSFV. View Full-Text
Keywords: classical swine fever virus; virus–host interactions; NS5A; eukaryotic elongation factor 1A; internal ribosome entry site classical swine fever virus; virus–host interactions; NS5A; eukaryotic elongation factor 1A; internal ribosome entry site
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MDPI and ACS Style

Li, S.; Feng, S.; Wang, J.-H.; He, W.-R.; Qin, H.-Y.; Dong, H.; Li, L.-F.; Yu, S.-X.; Li, Y.; Qiu, H.-J. eEF1A Interacts with the NS5A Protein and Inhibits the Growth of Classical Swine Fever Virus. Viruses 2015, 7, 4563-4581.

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