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Viruses 2015, 7(7), 3954-3973; doi:10.3390/v7072807

Synthetic RNAs Mimicking Structural Domains in the Foot-and-Mouth Disease Virus Genome Elicit a Broad Innate Immune Response in Porcine Cells Triggered by RIG-I and TLR Activation

1
Centro de Investigación en Sanidad Animal, CISA-INIA, Valdeolmos, Madrid 28130, Spain
2
Centro de Biología Molecular Severo Ochoa (CISC-UAM), Cantoblanco, Madrid 28049, Spain
3
Dpto. de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña Km 7.5, Madrid 28040, Spain
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Eric O. Freed
Received: 31 March 2015 / Revised: 8 July 2015 / Accepted: 10 July 2015 / Published: 17 July 2015
(This article belongs to the Special Issue Advances in Gene Technology and Resistance to Viruses)
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Abstract

The innate immune system is the first line of defense against viral infections. Exploiting innate responses for antiviral, therapeutic and vaccine adjuvation strategies is being extensively explored. We have previously described, the ability of small in vitro RNA transcripts, mimicking the sequence and structure of different domains in the non-coding regions of the foot-and-mouth disease virus (FMDV) genome (ncRNAs), to trigger a potent and rapid innate immune response. These synthetic non-infectious molecules have proved to have a broad-range antiviral activity and to enhance the immunogenicity of an FMD inactivated vaccine in mice. Here, we have studied the involvement of pattern-recognition receptors (PRRs) in the ncRNA-induced innate response and analyzed the antiviral and cytokine profiles elicited in swine cultured cells, as well as peripheral blood mononuclear cells (PBMCs). View Full-Text
Keywords: antivirals; non-coding RNA; viral sensors; innate immunity; foot-and-mouth disease virus antivirals; non-coding RNA; viral sensors; innate immunity; foot-and-mouth disease virus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Borrego, B.; Rodríguez-Pulido, M.; Revilla, C.; Álvarez, B.; Sobrino, F.; Domínguez, J.; Sáiz, M. Synthetic RNAs Mimicking Structural Domains in the Foot-and-Mouth Disease Virus Genome Elicit a Broad Innate Immune Response in Porcine Cells Triggered by RIG-I and TLR Activation. Viruses 2015, 7, 3954-3973.

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