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Viruses 2015, 7(7), 3816-3834; doi:10.3390/v7072799

Functional Characterization of Cucumis metuliferus Proteinase Inhibitor Gene (CmSPI) in Potyviruses Resistance

Agronomy Department National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan
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Academic Editor: Thomas Hohn
Received: 27 April 2015 / Revised: 2 July 2015 / Accepted: 3 July 2015 / Published: 9 July 2015
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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Abstract

Proteinase inhibitors are ubiquitous proteins that block the active center or interact allosterically with proteinases and are involved in plant physiological processes and defense responses to biotic and abiotic stresses. The CmSPI gene identified from Cucumis metuliferus encodes a serine type PI (8 kDa) that belongs to potato I type family. To evaluate the effect of silencing CmSPI gene on Papaya ringspot virus resistance, RNA interference (RNAi) with an inter-space hairpin RNA (ihpRNA) construct was introduced into a PRSV-resistant C. metuliferus line. CmSPI was down-regulated in CmSPI RNAi transgenic lines in which synchronously PRSV symptoms were evident at 21 day post inoculation. Alternatively, heterogeneous expression of CmSPI in Nicotiana benthamiana was also conducted and showed that CmSPI can provide resistance to Potato virus Y, another member of Potyvirus, in transgenic N. benthamiana lines. This study demonstrated that CmSPI plays an important role in resistant function against potyviruses in C. metuliferus and N. benthamiana. View Full-Text
Keywords: Proteinase inhibitors (PIs); Cucumis metuliferus; Papaya ringspot virus (PRSV); RNA interference (RNAi); Potato virus Y (PVY) Proteinase inhibitors (PIs); Cucumis metuliferus; Papaya ringspot virus (PRSV); RNA interference (RNAi); Potato virus Y (PVY)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lin, C.-W.; Su, M.-H.; Lin, Y.-T.; Chung, C.-H.; Ku, H.-M. Functional Characterization of Cucumis metuliferus Proteinase Inhibitor Gene (CmSPI) in Potyviruses Resistance. Viruses 2015, 7, 3816-3834.

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