Next Article in Journal
Viral Membrane Channels: Role and Function in the Virus Life Cycle
Previous Article in Journal
RNA Viruses and RNAi: Quasispecies Implications for Viral Escape
Article Menu

Export Article

Open AccessArticle
Viruses 2015, 7(6), 3241-3260; doi:10.3390/v7062769

Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Eric O. Freed
Received: 21 April 2015 / Revised: 12 June 2015 / Accepted: 15 June 2015 / Published: 19 June 2015
(This article belongs to the Section Animal Viruses)
View Full-Text   |   Download PDF [1347 KB, uploaded 19 June 2015]   |  

Abstract

Human immunodeficiency virus (HIV)-1 has a unique integration profile in the human genome relative to murine and avian retroviruses. Equine infectious anemia virus (EIAV) is another well-studied lentivirus that can also be used as a promising retro-transfection vector, but its integration into its native host has not been characterized. In this study, we mapped 477 integration sites of the EIAV strain EIAVFDDV13 in fetal equine dermal (FED) cells during in vitro infection. Published integration sites of EIAV and HIV-1 in the human genome were also analyzed as references. Our results demonstrated that EIAVFDDV13 tended to integrate into genes and AT-rich regions, and it avoided integrating into transcription start sites (TSS), which is consistent with EIAV and HIV-1 integration in the human genome. Notably, the integration of EIAVFDDV13 favored long interspersed elements (LINEs) and DNA transposons in the horse genome, whereas the integration of HIV-1 favored short interspersed elements (SINEs) in the human genome. The chromosomal environment near LINEs or DNA transposons potentially influences viral transcription and may be related to the unique EIAV latency states in equids. The data on EIAV integration in its natural host will facilitate studies on lentiviral infection and lentivirus-based therapeutic vectors. View Full-Text
Keywords: equine infectious anemia virus; integration sites; chromosomes; RefSeq genes; repetitive elements equine infectious anemia virus; integration sites; chromosomes; RefSeq genes; repetitive elements
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Liu, Q.; Wang, X.-F.; Ma, J.; He, X.-J.; Wang, X.-J.; Zhou, J.-H. Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome. Viruses 2015, 7, 3241-3260.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top