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Viruses 2015, 7(12), 6141-6151; doi:10.3390/v7122930

Silencing of a Germin-Like Protein Gene (CchGLP) in Geminivirus-Resistant Pepper (Capsicum chinense Jacq.) BG-3821 Increases Susceptibility to Single and Mixed Infections by Geminiviruses PHYVV and PepGMV

1
C.A. Ingeniería de Biosistemas, Facultad de Ingeniería-Campus Amazcala, Carretera a Chichimequillas, Km. 1, S/N, El Marques, Queretaro C.P. 76229, Mexico
2
Instituto Tecnológico de Roque, Departamento de Ingeniería en Industrias Alimentarias, Km. 8 Carr. Celaya-J. Rosas, Roque, Celaya, Gto C.P. 38110, Mexico
3
Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados (CINVESTAV)-Unidad Irapuato, Carretera Irapuato-Leon, Km 9.6, Libramiento norte, Irapuato, Guanajuato A.P. 629, Mexico
4
Departamento de Ingeniería Bioquímica, Instituto Tecnológico de Celaya, Ave. Tecnológico y A, Garcia-Cubas, S/N, Col. FOVISSSTE, Celaya, Gto A.P. 57, Mexico
These authors contributed equally to this work.
Present address: Universidad de Guanajuato, Campus Celaya-Salvatierra, División de Ciencias de la Salud e Ingenierías, Departamento de Ingeniería Agroindustrial, Programa de Ingeniería en Biotecnología, Av. Mutualismo Esq. Prolongación Río Lerma S/N, Celaya, Gto. C.P. 38060, Mexico; Tel.: +52-461-614-6440.
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas Hohn
Received: 11 September 2015 / Revised: 11 November 2015 / Accepted: 12 November 2015 / Published: 25 November 2015
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
View Full-Text   |   Download PDF [2196 KB, uploaded 25 November 2015]   |  

Abstract

Germin-like proteins (GLPs) are encoded by a family of genes found in all plants, and in terms of function, the GLPs are implicated in the response of plants to biotic and abiotic stresses. CchGLP is a gene encoding a GLP identified in a geminivirus-resistant Capsicum chinense Jacq accession named BG-3821, and it is important in geminivirus resistance when transferred to susceptible tobacco in transgenic experiments. To characterize the role of this GLP in geminivirus resistance in the original accession from which this gene was identified, this work aimed at demonstrating the possible role of CchGLP in resistance to geminiviruses in Capsicum chinense Jacq. BG-3821. Virus-induced gene silencing studies using a geminiviral vector based in PHYVV component A, displaying that silencing of CchGLP in accession BG-3821, increased susceptibility to geminivirus single and mixed infections. These results suggested that CchGLP is an important factor for geminivirus resistance in C. chinense BG-3821 accession. View Full-Text
Keywords: VIGS; plant-virus interaction; resistance; Mn-SOD; transient expression; Germin-like proteins; J0101 VIGS; plant-virus interaction; resistance; Mn-SOD; transient expression; Germin-like proteins; J0101
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Mejía-Teniente, L.; Joaquin-Ramos, A.J.; Torres-Pacheco, I.; Rivera-Bustamante, R.F.; Guevara-Olvera, L.; Rico-García, E.; Guevara-Gonzalez, R.G. Silencing of a Germin-Like Protein Gene (CchGLP) in Geminivirus-Resistant Pepper (Capsicum chinense Jacq.) BG-3821 Increases Susceptibility to Single and Mixed Infections by Geminiviruses PHYVV and PepGMV. Viruses 2015, 7, 6141-6151.

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