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Viruses 2015, 7(11), 5875-5888; doi:10.3390/v7112912

Psoralen Inactivation of Viruses: A Process for the Safe Manipulation of Viral Antigen and Nucleic Acid

1
National Biodefense Analysis and Countermeasures Center (NBACC), Fort Detrick, MD 21702, USA
2
Naval Medical Research Center, Silver Spring, MD 20910, USA
3
University of Maryland, College Park, MD 20742, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Curt Hagedorn
Received: 22 September 2015 / Revised: 3 November 2015 / Accepted: 4 November 2015 / Published: 12 November 2015
(This article belongs to the Section Antivirals & Vaccines)
View Full-Text   |   Download PDF [1628 KB, uploaded 12 November 2015]   |  

Abstract

High consequence human pathogenic viruses must be handled at biosafety level 2, 3 or 4 and must be rendered non-infectious before they can be utilized for molecular or immunological applications at lower biosafety levels. Here we evaluate psoralen-inactivated Arena-, Bunya-, Corona-, Filo-, Flavi- and Orthomyxoviruses for their suitability as antigen in immunological processes and as template for reverse transcription PCR and sequencing. The method of virus inactivation using a psoralen molecule appears to have broad applicability to RNA viruses and to leave both the particle and RNA of the treated virus intact, while rendering the virus non-infectious. View Full-Text
Keywords: virus; inactivation; biocontainment; BSL-4; BSL-3; psoralen; Trizol LS; IFA; safety; biosafety; PCR; reverse transcription; sequencing; antibody; sterility virus; inactivation; biocontainment; BSL-4; BSL-3; psoralen; Trizol LS; IFA; safety; biosafety; PCR; reverse transcription; sequencing; antibody; sterility
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Schneider, K.; Wronka-Edwards, L.; Leggett-Embrey, M.; Walker, E.; Sun, P.; Ondov, B.; Wyman, T.H.; Rosovitz, M.; Bohn, S.S.; Burans, J.; Kochel, T. Psoralen Inactivation of Viruses: A Process for the Safe Manipulation of Viral Antigen and Nucleic Acid. Viruses 2015, 7, 5875-5888.

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