Next Article in Journal
HCV Drug Resistance Challenges in Japan: The Role of Pre-Existing Variants and Emerging Resistant Strains in Direct Acting Antiviral Therapy
Previous Article in Journal
The Role of Electron Microscopy in Studying the Continuum of Changes in Membranous Structures during Poliovirus Infection
Article Menu

Export Article

Open AccessCommunication
Viruses 2015, 7(10), 5319-5327; doi:10.3390/v7102873

Multi-Modal Imaging with a Toolbox of Influenza AReporter Viruses

1
Medical Microbiology and Immunology, University of Wisconsin Madison, Madison, WI 53706, USA
2
Microbiology Doctoral Training Program, University of Wisconsin Madison, Madison, WI 53706, USA
3
Carbone Cancer Center, University of Wisconsin Madison, Madison, WI 53706, USA
4
GlaxoSmithKline, Antiviral Discovery Performance Unit, 5 Moore Drive, Research Triangle Park, NC 27709, USA
5
PerkinElmer, 68 Elm Street, Hopkinton, MA 01748, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Eric O. Freed
Received: 4 September 2015 / Revised: 23 September 2015 / Accepted: 24 September 2015 / Published: 13 October 2015
(This article belongs to the Section Animal Viruses)
View Full-Text   |   Download PDF [3703 KB, uploaded 13 October 2015]   |  

Abstract

Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography–computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies. View Full-Text
Keywords: influenza virus; reporter virus; multi-modal imaging; multiplicity reactivation influenza virus; reporter virus; multi-modal imaging; multiplicity reactivation
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Tran, V.; Poole, D.S.; Jeffery, J.J.; Sheahan, T.P.; Creech, D.; Yevtodiyenko, A.; Peat, A.J.; Francis, K.P.; You, S.; Mehle, A. Multi-Modal Imaging with a Toolbox of Influenza AReporter Viruses. Viruses 2015, 7, 5319-5327.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top