Next Article in Journal
KSHV Reactivation and Novel Implications of Protein Isomerization on Lytic Switch Control
Previous Article in Journal
Immune Memory to Sudan Virus: Comparison between Two Separate Disease Outbreaks
Article Menu

Export Article

Open AccessArticle
Viruses 2015, 7(1), 52-71; doi:10.3390/v7010052

Herpes Simplex Virus 1 Us3 Deletion Mutant is Infective Despite Impaired Capsid Translocation to the Cytoplasm

1
Institute of Veterinar Anatomy, Winterthurerstrasse 260, CH-8057 Zürich, Switzerland
2
Institute of Virology, Winterthurerstrasse 266a, CH-8057 Zürich, Switzerland
3
Center for Microscopy and Image Analysis, Winterthurerstrasse 190,CH-8057 Zürich, Switzerland
*
Author to whom correspondence should be addressed.
Academic Editor: Eric O. Freed
Received: 24 November 2014 / Accepted: 30 December 2014 / Published: 12 January 2015
View Full-Text   |   Download PDF [2645 KB, uploaded 12 May 2015]   |  

Abstract

Herpes simplex virus 1 (HSV-1) capsids are assembled in the nucleus bud at the inner nuclear membrane into the perinuclear space, acquiring envelope and tegument. In theory, these virions are de-enveloped by fusion of the envelope with the outer nuclear membrane and re-enveloped by Golgi membranes to become infective. Us3 enables the nucleus to cytoplasm capsid translocation. Nevertheless, Us3 is not essential for the production of infective progeny viruses. Determination of phenotype distribution by quantitative electron microscopy, and calculation per mean nuclear or cell volume revealed the following: (i) The number of R7041(∆US3) capsids budding at the inner nuclear membrane was significantly higher than that of wild type HSV-1; (ii) The mean number of R7041(∆US3) virions per mean cell volume was 2726, that of HSV-1 virions 1460 by 24 h post inoculation; (iii) 98% of R7041(∆US3) virions were in the perinuclear space; (iv) The number of R7041(∆US3) capsids in the cytoplasm, including those budding at Golgi membranes, was significantly reduced. Cell associated R7041(∆US3) yields were 2.37 × 108 and HSV-1 yields 1.57 × 108 PFU/mL by 24 h post inoculation. We thus conclude that R7041(∆US3) virions, which acquire envelope and tegument by budding at the inner nuclear membrane into the perinuclear space, are infective. View Full-Text
Keywords: HSV-1; Us3 protein kinase; envelopment; morphogenesis; electron microscopy; morphometry HSV-1; Us3 protein kinase; envelopment; morphogenesis; electron microscopy; morphometry
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wild, P.; Leisinger, S.; de Oliveira, A.P.; Schraner, E.M.; Kaech, A.; Ackermann, M.; Tobler, K. Herpes Simplex Virus 1 Us3 Deletion Mutant is Infective Despite Impaired Capsid Translocation to the Cytoplasm. Viruses 2015, 7, 52-71.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top