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Viruses 2014, 6(3), 1135-1148; doi:10.3390/v6031135

Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts

Department of Molecular Medicine, Mayo Clinic, Rochester, MN 55905 USA
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Received: 12 December 2013 / Revised: 21 February 2014 / Accepted: 25 February 2014 / Published: 10 March 2014
(This article belongs to the Section Animal Viruses)
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Abstract

Previously we reported that nuclear export of both unspliced and spliced murine leukemia virus (MLV) transcripts depends on the nuclear export factor (NXF1) pathway. Although the mRNA export complex TREX, which contains Aly/REF, UAP56, and the THO complex, is involved in the NXF1-mediated nuclear export of cellular mRNAs, its contribution to the export of MLV mRNA transcripts remains poorly understood. Here, we studied the involvement of TREX components in the export of MLV transcripts. Depletion of UAP56, but not Aly/REF, reduced the level of both unspliced and spliced viral transcripts in the cytoplasm. Interestingly, depletion of THO components, including THOC5 and THOC7, affected only unspliced viral transcripts in the cytoplasm. Moreover, the RNA immunoprecipitation assay showed that only the unspliced viral transcript interacted with THOC5. These results imply that MLV requires UAP56, THOC5 and THOC7, in addition to NXF1, for nuclear export of viral transcripts. Given that naturally intronless mRNAs, but not bulk mRNAs, require THOC5 for nuclear export, it is plausible that THOC5 plays a key role in the export of unspliced MLV transcripts. View Full-Text
Keywords: gammaretrovirus; MLV; UAP56; THOC5; THOC7; TREX; RNA nuclear export gammaretrovirus; MLV; UAP56; THOC5; THOC7; TREX; RNA nuclear export
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Sakuma, T.; Tonne, J.M.; Ikeda, Y. Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts. Viruses 2014, 6, 1135-1148.

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