Viruses 2013, 5(3), 928-953; doi:10.3390/v5030928
Article

Protective Effect of Surfactant Protein D in Pulmonary Vaccinia Virus Infection: Implication of A27 Viral Protein

1email, 2email, 3email, 4email, 1email and 1,* email
Received: 11 February 2013; in revised form: 11 March 2013 / Accepted: 12 March 2013 / Published: 21 March 2013
(This article belongs to the Section Animal Viruses)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Vaccinia virus (VACV) was used as a surrogate of variola virus (VARV) (genus Orthopoxvirus), the causative agent of smallpox, to study Orthopoxvirus infection. VARV is principally transmitted between humans by aerosol droplets. Once inhaled, VARV first infects the respiratory tract where it could encounter surfactant components, such as soluble pattern recognition receptors. Surfactant protein D (SP-D), constitutively present in the lining fluids of the respiratory tract, plays important roles in innate host defense against virus infection. We investigated the role of SP-D in VACV infection and studied the A27 viral protein involvement in the interaction with SP-D. Interaction between SP-D and VACV caused viral inhibition in a lung cell model. Interaction of SP-D with VACV was mediated by the A27 viral protein. Binding required Ca2+ and interactions were blocked in the presence of excess of SP-D saccharide ligands. A27, which lacks glycosylation, directly interacted with SP-D. The interaction between SP-D and the viral particle was also observed using electron microscopy. Infection of mice lacking SP-D (SP-D-/-) resulted in increased mortality compared to SP-D+/+ mice. Altogether, our data show that SP-D participates in host defense against the vaccinia virus infection and that the interaction occurs with the viral surface protein A27.
Keywords: orthopoxvirus; surfactant protein D; vaccinia virus; A27 protein; pulmonary infection; innate immunity; surfactant protein A; KO mice; Ca2+; sugars
PDF Full-text Download PDF Full-Text [931 KB, Updated Version, uploaded 25 March 2013 11:39 CET]
The original version is still available [935 KB, uploaded 21 March 2013 10:33 CET]

Export to BibTeX |
EndNote


MDPI and ACS Style

Perino, J.; Thielens, N.M.; Crouch, E.; Spehner, D.; Crance, J.-M.; Favier, A.-L. Protective Effect of Surfactant Protein D in Pulmonary Vaccinia Virus Infection: Implication of A27 Viral Protein. Viruses 2013, 5, 928-953.

AMA Style

Perino J, Thielens NM, Crouch E, Spehner D, Crance J-M, Favier A-L. Protective Effect of Surfactant Protein D in Pulmonary Vaccinia Virus Infection: Implication of A27 Viral Protein. Viruses. 2013; 5(3):928-953.

Chicago/Turabian Style

Perino, Julien; Thielens, Nicole M.; Crouch, Erika; Spehner, Danièle; Crance, Jean-Marc; Favier, Anne-Laure. 2013. "Protective Effect of Surfactant Protein D in Pulmonary Vaccinia Virus Infection: Implication of A27 Viral Protein." Viruses 5, no. 3: 928-953.

Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert