Next Article in Journal
Directional Spread of Alphaherpesviruses in the Nervous System
Previous Article in Journal
Low Copper and High Manganese Levels in Prion Protein Plaques
Viruses 2013, 5(2), 663-677; doi:10.3390/v5020663
Article

Predicted Peptides from Non-Structural Proteins of Porcine Reproductive and Respiratory Syndrome Virus Are Able to Induce IFN-γ and IL-10

1,2
,
2
,
2,3, 4
,
1
 and
2,5,*
1 Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo A.C (CIAD), Carretera a la Victoria Km 0.6, C.P. 83304 Hermosillo, Sonora, Mexico 2 Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain 3 Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Córdoba, Córdoba, Spain 4 Department of Pathobiology, Institute of Immunology, University of Veterinary Medicine Vienna, Vienna, 1210, Austria 5 Departament de Sanitat i d'Anatomia Animals, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain
* Author to whom correspondence should be addressed.
Received: 26 December 2012 / Revised: 7 February 2013 / Accepted: 9 February 2013 / Published: 11 February 2013
(This article belongs to the Section Animal Viruses)
View Full-Text   |   Download PDF [415 KB, uploaded 12 May 2015]
SciFeed

Abstract

This work describes peptides from non-structural proteins (nsp) of porcine reproductive and respiratory syndrome virus (PRRSV) predicted as potential T cell epitopes by bioinfornatics and tested for their ability to induce IFN-γ and IL-10 responses. Pigs immunized with either genotype 1 or genotype 2 PRRSV attenuated vaccines (n=5/group) and unvaccinated pigs (n = 4) were used to test the peptides. Swine leukocyte antigen haplotype of each pig was also determined. Pigs were initially screened for IFN-γ responses (ELISPOT) and three peptides were identified; two of them in non-conserved segments of nsp2 and nsp5 and the other in a conserved region of nsp5 peptide. Then, peptides were screened for IL-10 inducing properties. Six peptides were found to induce IL-10 release in PBMC and some of them were also able to inhibit IFN-γ responses on PHA-stimulated cells. Interestingly, the IFN-γ low responder pigs against PRRSV were mostly homozygous for their SLA haplotypes. In conclusion, these results indicate that nsp of PRRSV contain T-cell epitopes inducing IFN-γ responses as well as IL-10 inducing segments with inhibitory capabilities.
Keywords: PRRSV; non-structural proteins; epitopes; interferon gamma; IL-10 PRRSV; non-structural proteins; epitopes; interferon gamma; IL-10
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Burgara-Estrella, A.; Díaz, I.; Rodríguez-Gómez, I.M.; Essler, S.E.; Hernández, J.; Mateu, E. Predicted Peptides from Non-Structural Proteins of Porcine Reproductive and Respiratory Syndrome Virus Are Able to Induce IFN-γ and IL-10. Viruses 2013, 5, 663-677.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert