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• Burgara-Estrella, A
• Díaz, I
• Rodríguez-Gómez, IM.
• Essler, SE.
• Hernández, J
• Mateu, E
• [+] on Google Scholar
• Burgara-Estrella, A
• Díaz, I
• Rodríguez-Gómez, IM.
• Essler, SE.
• Hernández, J
• Mateu, E
• [+] on PubMed
• Burgara-Estrella, A
• Díaz, I
• Rodríguez-Gómez, IM.
• Essler, SE.
• Hernández, J
• Mateu, E

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Viruses 2013, 5(2), 663-677; doi:10.3390/v5020663

Article

Predicted Peptides from Non-Structural Proteins of Porcine Reproductive and Respiratory Syndrome Virus Are Able to Induce IFN-γ and IL-10

Alexel Burgara-Estrella ^1,2 , Ivan Díaz ² , Irene M. Rodríguez-Gómez ^2,3 , Sabine E. Essler ⁴ , Jesús Hernández ¹  and Enric Mateu ^2,5,*

¹ Laboratorio de Inmunología, Centro de Investigación en Alimentación y Desarrollo A.C (CIAD), Carretera a la Victoria Km 0.6, C.P. 83304 Hermosillo, Sonora, Mexico ² Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain ³ Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Córdoba, Córdoba, Spain ⁴ Department of Pathobiology, Institute of Immunology, University of Veterinary Medicine Vienna, Vienna, 1210, Austria ⁵ Departament de Sanitat i d'Anatomia Animals, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain

* Author to whom correspondence should be addressed.

Received: 26 December 2012; in revised form: 7 February 2013 / Accepted: 9 February 2013 / Published: 11 February 2013

Download PDF Full-Text [415 KB, uploaded 12 February 2013 09:06 CET]

Abstract: This work describes peptides from non-structural proteins (nsp) of porcine reproductive and respiratory syndrome virus (PRRSV) predicted as potential T cell epitopes by bioinfornatics and tested for their ability to induce IFN-γ and IL-10 responses. Pigs immunized with either genotype 1 or genotype 2 PRRSV attenuated vaccines (n=5/group) and unvaccinated pigs (n = 4) were used to test the peptides. Swine leukocyte antigen haplotype of each pig was also determined. Pigs were initially screened for IFN-γ responses (ELISPOT) and three peptides were identified; two of them in non-conserved segments of nsp2 and nsp5 and the other in a conserved region of nsp5 peptide. Then, peptides were screened for IL-10 inducing properties. Six peptides were found to induce IL-10 release in PBMC and some of them were also able to inhibit IFN-γ responses on PHA-stimulated cells. Interestingly, the IFN-γ low responder pigs against PRRSV were mostly homozygous for their SLA haplotypes. In conclusion, these results indicate that nsp of PRRSV contain T-cell epitopes inducing IFN-γ responses as well as IL-10 inducing segments with inhibitory capabilities.

Keywords: PRRSV; non-structural proteins; epitopes; interferon gamma; IL-10

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