Viruses 2013, 5(10), 2531-2545; doi:10.3390/v5102531

Oligopeptide M13 Phage Display in Pathogen Research

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Received: 17 September 2013; in revised form: 8 October 2013 / Accepted: 9 October 2013 / Published: 16 October 2013
(This article belongs to the Special Issue Phage Display in Virus Research)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Phage display has become an established, widely used method for selection of peptides, antibodies or alternative scaffolds. The use of phage display for the selection of antigens from genomic or cDNA libraries of pathogens which is an alternative to the classical way of identifying immunogenic proteins is not well-known. In recent years several new applications for oligopeptide phage display in disease related fields have been developed which has led to the identification of various new antigens. These novel identified immunogenic proteins provide new insights into host pathogen interactions and can be used for the development of new diagnostic tests and vaccines. In this review we focus on the M13 oligopeptide phage display system for pathogen research but will also give examples for lambda phage display and for applications in other disease related fields. In addition, a detailed technical work flow for the identification of immunogenic oligopeptides using the pHORF system is given. The described identification of immunogenic proteins of pathogens using oligopeptide phage display can be linked to antibody phage display resulting in a vaccine pipeline.
Keywords: phage display; ORF selection; immunogenic proteins; vaccine; oligopeptide phage display; diagnostic; phage display libraries; genome libraries; antigen discovery
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MDPI and ACS Style

Kügler, J.; Zantow, J.; Meyer, T.; Hust, M. Oligopeptide M13 Phage Display in Pathogen Research. Viruses 2013, 5, 2531-2545.

AMA Style

Kügler J, Zantow J, Meyer T, Hust M. Oligopeptide M13 Phage Display in Pathogen Research. Viruses. 2013; 5(10):2531-2545.

Chicago/Turabian Style

Kügler, Jonas; Zantow, Jonas; Meyer, Torsten; Hust, Michael. 2013. "Oligopeptide M13 Phage Display in Pathogen Research." Viruses 5, no. 10: 2531-2545.

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