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Viruses 2011, 3(4), 331-335; doi:10.3390/v3040331
Commentary

Initiation of HIV Reverse Transcription: Is Enzyme Flipping Required?

1,2
1 Department of Microbiology and Immunology, McGill University, Duff Medical Building (D-6), 3775 University St., Montreal, QC, H3A 2B4, Canada 2 Department of Biochemistry, McGill University, Montreal, QC, H3G 1Y6, Canada
Received: 9 March 2011 / Revised: 1 April 2011 / Accepted: 1 April 2011 / Published: 12 April 2011
(This article belongs to the Section Editorial)
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Abstract

Liu and colleagues have recently studied dynamic changes in the orientation of HIV reverse transcriptase (RT) on its nucleic acid substrate during initiation of DNA synthesis. The authors employed a single molecule FRET assay and revealed the existence of an equilibrium between polymerase-competent and “flipped” polymerase-incompetent orientations. RT flipping correlates with enzyme pausing during initiation, while the transition to the processive elongation phase correlates with increases in the population of polymerase-competent complexes. The potential biological significance of these findings is discussed in this commentary in lieu of the entire process of reverse transcription.
Keywords: HIV; reverse transcription; initiation HIV; reverse transcription; initiation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Götte, M. Initiation of HIV Reverse Transcription: Is Enzyme Flipping Required? Viruses 2011, 3, 331-335.

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