Viruses of Eukaryotic Algae: Diversity, Methods for Detection, and Future Directions
AbstractThe scope for ecological studies of eukaryotic algal viruses has greatly improved with the development of molecular and bioinformatic approaches that do not require algal cultures. Here, we review the history and perceived future opportunities for research on eukaryotic algal viruses. We begin with a summary of the 65 eukaryotic algal viruses that are presently in culture collections, with emphasis on shared evolutionary traits (e.g., conserved core genes) of each known viral type. We then describe how core genes have been used to enable molecular detection of viruses in the environment, ranging from PCR-based amplification to community scale “-omics” approaches. Special attention is given to recent studies that have employed network-analyses of -omics data to predict virus-host relationships, from which a general bioinformatics pipeline is described for this type of approach. Finally, we conclude with acknowledgement of how the field of aquatic virology is adapting to these advances, and highlight the need to properly characterize new virus-host systems that may be isolated using preliminary molecular surveys. Researchers can approach this work using lessons learned from the Chlorella virus system, which is not only the best characterized algal-virus system, but is also responsible for much of the foundation in the field of aquatic virology. View Full-Text
- Supplementary File 1:
ZIP-Document (ZIP, 240 KB)
Share & Cite This Article
Coy, S.R.; Gann, E.R.; Pound, H.L.; Short, S.M.; Wilhelm, S.W. Viruses of Eukaryotic Algae: Diversity, Methods for Detection, and Future Directions. Viruses 2018, 10, 487.
Coy SR, Gann ER, Pound HL, Short SM, Wilhelm SW. Viruses of Eukaryotic Algae: Diversity, Methods for Detection, and Future Directions. Viruses. 2018; 10(9):487.Chicago/Turabian Style
Coy, Samantha R.; Gann, Eric R.; Pound, Helena L.; Short, Steven M.; Wilhelm, Steven W. 2018. "Viruses of Eukaryotic Algae: Diversity, Methods for Detection, and Future Directions." Viruses 10, no. 9: 487.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.