Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate
AbstractHere, we report the anti-human immunodeficiency virus (HIV) potency and underlying mechanisms of a Keggin polyoxometalate (PT-1, K6HPTi2W10O40). Our findings showed that PT-1 exhibited highly potent effects against a diverse group of HIV type 1 (HIV-1) strains and displayed low cytotoxicity and genotoxicity. The time-addition assay revealed that PT-1 acted at an early stage of infection, and these findings were supported by the observation that PT-1 had more potency against Env-pseudotyped virus than vesicular stomatitis virus glycoprotein (VSVG) pseudotyped virus. Surface plasmon resonance binding assays and flow cytometry analysis showed that PT-1 blocked the gp120 binding site in the CD4 receptor. Moreover, PT-1 bound directly to gp41 NHR (N36 peptide), thereby interrupting the core bundle formation of gp41. In conclusion, our data suggested that PT-1 may be developed as a new anti-HIV-1 agent through its effects on entry inhibition. View Full-Text
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Wang, X.; Wang, J.; Zhang, W.; Li, B.; Zhu, Y.; Hu, Q.; Yang, Y.; Zhang, X.; Yan, H.; Zeng, Y. Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate. Viruses 2018, 10, 265.
Wang X, Wang J, Zhang W, Li B, Zhu Y, Hu Q, Yang Y, Zhang X, Yan H, Zeng Y. Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate. Viruses. 2018; 10(5):265.Chicago/Turabian Style
Wang, Xiaoli; Wang, Jiao; Zhang, Wenmei; Li, Boye; Zhu, Ying; Hu, Qin; Yang, Yishu; Zhang, Xiaoguang; Yan, Hong; Zeng, Yi. 2018. "Inhibition of Human Immunodeficiency Virus Type 1 Entry by a Keggin Polyoxometalate." Viruses 10, no. 5: 265.
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