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Viruses 2018, 10(5), 260; https://doi.org/10.3390/v10050260

Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections

Sidney Laboratory, Centre for Plant Health, Canadian Food Inspection Agency, 8801 East Saanich Road, North Saanich, BC V8L 1H3, Canada
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Received: 30 March 2018 / Revised: 27 April 2018 / Accepted: 11 May 2018 / Published: 15 May 2018
(This article belongs to the Special Issue Fruit Tree Viruses and Viroids)
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Abstract

Blackcurrant leaf chlorosis associated virus (BCLCaV) was detected recently by next-generation sequencing (NGS) and a new and distinct species in the genus Idaeovirus was proposed. Analysis of NGS-derived paired-end reads revealed the existence of bridge reads encompassing the 3′-terminus and 5′-terminus of RNA-2 or RNA-3 of BCLCaV. The full RNA-2 or RNA-3 could be amplified using outward facing or abutting primers; also, RNA-2/RNA-3 could be detected even after three consecutive RNase R enzyme treatments, with denaturation at 95 °C preceding each digestion. Evidence was obtained indicating that there are circular forms of BCLCaV RNA-2 and RNA-3. View Full-Text
Keywords: Idaeovirus; blackcurrant leaf chlorosis associated virus; next-generation sequencing (NGS); bridge reads; abutting primers; RNase R digestion; circular RNA; concatenated RNA Idaeovirus; blackcurrant leaf chlorosis associated virus; next-generation sequencing (NGS); bridge reads; abutting primers; RNase R digestion; circular RNA; concatenated RNA
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James, D.; Phelan, J.; Sanderson, D. Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections. Viruses 2018, 10, 260.

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