Ostreid Herpesvirus-1 Infects Specific Hemocytes in Ark Clam, Scapharca broughtonii
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ark Clams
2.2. Experimental OsHV-1 Infection and Sample Collection
2.3. Quantification of OsHV-1 Load by RT-qPCR
2.4. Flow Cytometry Analysis
2.5. Hemocyte Morphologic Observation
2.6. Transmission Electron Microscopy (TEM) Examination
3. Results
3.1. OsHV-1 Load in Different Ark Clam Tissues
3.2. Characterization of Different Types of Ark Clam Hemocytes
3.3. Identification of OsHV-1 Infected Hemocytes by TEM
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Xin, L.; Li, C.; Bai, C.; Wang, C. Ostreid Herpesvirus-1 Infects Specific Hemocytes in Ark Clam, Scapharca broughtonii. Viruses 2018, 10, 529. https://doi.org/10.3390/v10100529
Xin L, Li C, Bai C, Wang C. Ostreid Herpesvirus-1 Infects Specific Hemocytes in Ark Clam, Scapharca broughtonii. Viruses. 2018; 10(10):529. https://doi.org/10.3390/v10100529
Chicago/Turabian StyleXin, Lusheng, Chen Li, Changming Bai, and Chongming Wang. 2018. "Ostreid Herpesvirus-1 Infects Specific Hemocytes in Ark Clam, Scapharca broughtonii" Viruses 10, no. 10: 529. https://doi.org/10.3390/v10100529