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Forests 2017, 8(6), 196; doi:10.3390/f8060196

Evaluation of a New Temporary Immersion Bioreactor System for Micropropagation of Cultivars of Eucalyptus, Birch and Fir

1
Department of Forest Genetics and Plant Physiology, Umeå Plant Science Center (UPSC), Swedish University of Agricultural Sciences (SLU), 901 83 Umeå, Sweden
2
SweTree Technologies AB, P.O Box 4095, 904 03 Umeå, Sweden
3
Institut für Pflanzenkultur e.K., Solkau 2, 29465 Schnega, Germany
4
G.W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 500 Tenth Street NW, 30332-0620 Atlanta, Georgia
*
Author to whom correspondence should be addressed.
Academic Editor: Jason A. Holliday
Received: 12 May 2017 / Revised: 31 May 2017 / Accepted: 31 May 2017 / Published: 3 June 2017
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Abstract

The use of liquid instead of solid culture medium for the micropropagation of plants offers advantages such as better access to medium components and scalability through possible automation of the processes. The objective of this work was to compare a new temporary immersion bioreactor (TIB) to solid medium culture for the micropropagation of a selection of tree species micropropagated for commercial use: Nordmann fir (Abies nordmanniana (Steven) Spach), Eucalyptus (E. grandis x E. urophylla), Downy birch (Betula pubescens Ehrh), and Curly birch (Betula pendula var. carelica). Cultivation of explants in the TIB resulted in a significant increase of multiplication rate and fresh weight of Eucalyptus and B. pendula, but not Betula pubescens. In addition, the fresh weight of embryogenic tissue and the maturation frequency of somatic embryos increased significantly when an embryogenic cell line of A. nordmanniana was cultivated in the TIB compared to solid culture medium. These results demonstrate the potential for scaling up and automating micropropagation by shoot multiplication and somatic embryogenesis in commercial tree species using a temporary immersion bioreactor. View Full-Text
Keywords: forest trees; temporary immersion; somatic embryogenesis; micropropagation forest trees; temporary immersion; somatic embryogenesis; micropropagation
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MDPI and ACS Style

Businge, E.; Trifonova, A.; Schneider, C.; Rödel, P.; Egertsdotter, U. Evaluation of a New Temporary Immersion Bioreactor System for Micropropagation of Cultivars of Eucalyptus, Birch and Fir. Forests 2017, 8, 196.

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