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Forests 2017, 8(11), 458; https://doi.org/10.3390/f8110458

Phenotypical and Molecular Characterisation of Fusarium circinatum: Correlation with Virulence and Fungicide Sensitivity

1
Forest Research, Alice Holt Lodge, Farnham, Surrey GU10 4LH, UK
2
Instituto Agroforestal Mediterráneo, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain
*
Authors to whom correspondence should be addressed.
Academic Editor: Julio Javier Diez Casero
Received: 25 October 2017 / Revised: 6 November 2017 / Accepted: 16 November 2017 / Published: 21 November 2017
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Abstract

Fusarium circinatum, causing pine pitch canker, is one of the most damaging pathogens of Pinus species. This study investigated the use of phenotypical and molecular characteristics to delineate groups in a worldwide collection of isolates. The groups correlated with virulence and fungicide sensitivity, which were tested in a subset of isolates. Virulence tests of twenty isolates on P. radiata, P. sylvestris and P. pinaster demonstrated differences in host susceptibility, with P. radiata most susceptible and P. sylvestris least susceptible. Sensitivity to the fungicides fludioxonil and pyraclostrobin varied considerably between isolates from highly effective (half-maximal effective concentration (EC50) < 0.1 ppm) to ineffective (EC50 > 100 ppm). This study demonstrates the potential use of simply acquired phenotypical (cultural, morphological) and molecular metrics to gain a preliminary estimate of virulence and sensitivity to certain fungicides. It also highlights the necessity of including a range of isolates in fungicide tests and host susceptibility assays, particularly of relevance to tree breeding programmes. View Full-Text
Keywords: pine pitch canker; mating type; susceptibility; variation; Gibberella circinata; pyraclostrobin; fludioxonil pine pitch canker; mating type; susceptibility; variation; Gibberella circinata; pyraclostrobin; fludioxonil
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Mullett, M.; Pérez-Sierra, A.; Armengol, J.; Berbegal, M. Phenotypical and Molecular Characterisation of Fusarium circinatum: Correlation with Virulence and Fungicide Sensitivity. Forests 2017, 8, 458.

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