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Materials 2016, 9(9), 744; doi:10.3390/ma9090744

Electrochemical Hydrogel Lithography of Calcium-Alginate Hydrogels for Cell Culture

1
Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan
2
WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8579, Japan
*
Authors to whom correspondence should be addressed.
Academic Editors: Sandra Van Vlierberghe and Peter Dubruel
Received: 31 July 2016 / Revised: 12 August 2016 / Accepted: 22 August 2016 / Published: 31 August 2016
(This article belongs to the Special Issue Smart Hydrogels for (Bio)printing Applications)
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Abstract

Here we propose a novel electrochemical lithography methodology for fabricating calcium-alginate hydrogels having controlled shapes. We separated the chambers for Ca2+ production and gel formation with alginate with a semipermeable membrane. Ca2+ formed in the production chamber permeated through the membrane to fabricate a gel structure on the membrane in the gel formation chamber. When the calcium-alginate hydrogels were modified with collagen, HepG2 cells proliferated on the hydrogels. These results show that electrochemical hydrogel lithography is useful for cell culture. View Full-Text
Keywords: electrodeposition; electrochemical hydrogel lithography; calcium-alginate hydrogel; cell culture electrodeposition; electrochemical hydrogel lithography; calcium-alginate hydrogel; cell culture
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Ozawa, F.; Ino, K.; Shiku, H.; Matsue, T. Electrochemical Hydrogel Lithography of Calcium-Alginate Hydrogels for Cell Culture. Materials 2016, 9, 744.

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