Next Article in Journal
Experimental Study of Damage Evolution in Circular Stirrup-Confined Concrete
Previous Article in Journal
Integrated Utilization of Sewage Sludge and Coal Gangue for Cement Clinker Products: Promoting Tricalcium Silicate Formation and Trace Elements Immobilization
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle
Materials 2016, 9(4), 273; doi:10.3390/ma9040273

Label Free Poly(2,5-dimethoxyaniline)–Multi-Walled Carbon Nanotubes Impedimetric Immunosensor for Fumonisin B1 Detection

1
SensorLab, Department of Chemistry, University of the Western Cape Robert Sobukwe Road, Bellville 7535, Cape Town, South Africa
2
Department of Biological and Chemical Sciences, The University of the West Indies, Cave Hill Campus, Bridgetown BB11000, Barbados
*
Author to whom correspondence should be addressed.
Academic Editor: Dusan Losic
Received: 14 February 2016 / Revised: 12 March 2016 / Accepted: 24 March 2016 / Published: 7 April 2016
View Full-Text   |   Download PDF [4015 KB, uploaded 7 April 2016]   |  

Abstract

An impedimetric immunosensor for fumonisin B1 (FB1) was developed from a poly(2,5-dimethoxyaniline)-multi-walled carbon nanotube (PDMA-MWCNT) composite on the surface of glassy carbon electrode (GCE). The composite was prepared electrochemically and characterized using cyclic voltammetry. The preparation of the FB1 immunosensor involved the drop-coating of a bovine serum albumin mixture of the anti-fumonisin antibody (anti-Fms) onto the composite polymer-modified GCE. The electrochemical impedance spectroscopy (EIS) responses of the FB1 immunosensor (GCE/PDMA-MWCNT/anti-Fms) have a linear range of 7 to 49 ng·L−1, and the corresponding sensitivity and detection limits are 0.272 kΩ L·ng−1 and 3.8 pg·L−1, respectively. The limit of detection of the immunosensor for certified corn sample (i.e., certified reference material) is 0.014 ppm FB1, which is in excellent agreement with the value published by the vendors and significantly more accurate than that obtained with enzyme-linked immunosorbent assay (ELISA). View Full-Text
Keywords: fumonisin B1; anti-fumonisin antibody; immunosensor; poly(2,5-dimethoxyaniline)-multi-walled carbon nanotubes; certified reference materials; electrochemical impedance spectroscopy fumonisin B1; anti-fumonisin antibody; immunosensor; poly(2,5-dimethoxyaniline)-multi-walled carbon nanotubes; certified reference materials; electrochemical impedance spectroscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Masikini, M.; Williams, A.R.; Sunday, C.E.; Waryo, T.T.; Nxusani, E.; Wilson, L.; Qakala, S.; Bilibana, M.; Douman, S.; Jonnas, A.; Baker, P.G.L.; Iwuoha, E.I. Label Free Poly(2,5-dimethoxyaniline)–Multi-Walled Carbon Nanotubes Impedimetric Immunosensor for Fumonisin B1 Detection. Materials 2016, 9, 273.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top