Next Article in Journal
Macrophages, Foreign Body Giant Cells and Their Response to Implantable Biomaterials
Next Article in Special Issue
Integrating Microtissues in Nanofiber Scaffolds for Regenerative Nanomedicine
Previous Article in Journal
Influence of Flame Retardants on the Melt Dripping Behaviour of Thermoplastic Polymers
Previous Article in Special Issue
Smart Dressings Based on Nanostructured Fibers Containing Natural Origin Antimicrobial, Anti-Inflammatory, and Regenerative Compounds
Article Menu

Export Article

Open AccessArticle
Materials 2015, 8(9), 5647-5670; doi:10.3390/ma8095268

Biocompatibility and Stability of Polysaccharide Polyelectrolyte Complexes Aimed at Respiratory Delivery

1
CBMR—Centre for Biomedical Research, University of Algarve, Faculty of Sciences and Technology, Campus de Gambelas, Faro 8005-139, Portugal
2
CIQA—Algarve Chemistry Research Centre and Department of Chemistry and Pharmacy, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Faro 8005-139, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Nadia Jessel
Received: 1 July 2015 / Revised: 17 August 2015 / Accepted: 19 August 2015 / Published: 28 August 2015
(This article belongs to the Special Issue Therapeutics Delivery Systems for Regenerative Nanomedicine)
View Full-Text   |   Download PDF [2073 KB, uploaded 28 August 2015]   |  

Abstract

Chitosan (CS) and chondroitin sulfate (CHS) are natural polymers with demonstrated applicability in drug delivery, while nanoparticles are one of the most explored carriers for transmucosal delivery of biopharmaceuticals. In this work we have prepared CS/CHS nanoparticles and associated for the first time the therapeutic protein insulin. Fluorescein isothiocyanate bovine serum albumin (FITC-BSA) was also used to enable comparison of behaviors regarding differences in molecular weight (5.7 kDa versus 67 kDa). Nanoparticles of approximately 200 nm and positive zeta potential around +20 mV were obtained. These parameters remained stable for up to 1 month at 4 °C. Proteins were associated with efficiencies of more than 50%. The release of FITC-BSA in PBS pH 7.4 was more sustained (50% in 24 h) than that of insulin (85% in 24 h). The biocompatibility of nanoparticles was tested in Calu-3 and A549 cells by means of three different assays. The metabolic assay MTT, the determination of lactate dehydrogenase release, and the quantification of the inflammatory response generated by cell exposure to nanoparticles have indicated an absence of overt toxicity. Overall, the results suggest good indications on the application of CS/CHS nanoparticles in respiratory transmucosal protein delivery, but the set of assays should be widened to clarify obtained results. View Full-Text
Keywords: biocompatibility; chitosan; chondroitin sulfate; nasal delivery; polyelectrolyte complexes; polysaccharides; protein delivery; pulmonary delivery biocompatibility; chitosan; chondroitin sulfate; nasal delivery; polyelectrolyte complexes; polysaccharides; protein delivery; pulmonary delivery
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Rodrigues, S.; Cardoso, L.; da Costa, A.M.R.; Grenha, A. Biocompatibility and Stability of Polysaccharide Polyelectrolyte Complexes Aimed at Respiratory Delivery. Materials 2015, 8, 5647-5670.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Materials EISSN 1996-1944 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top