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Materials 2015, 8(4), 1636-1651; doi:10.3390/ma8041636

Characterization of Fibrin and Collagen Gels for Engineering Wound Healing Models

Multiscale in Mechanical and Biological Engineering (M2BE), Department of Mechanical Engineering, Aragon Institute of Engineering Research (I3A), Universidad de Zaragoza, Mariano Esquillor Street, 50018 Zaragoza, Spain
ITAINNOVA Instituto Tecnológico de Aragón, 7-8 María de Luna Street, 50018 Zaragoza, Spain
Author to whom correspondence should be addressed.
Academic Editor: Amir A. Zadpoor
Received: 27 February 2015 / Revised: 27 March 2015 / Accepted: 2 April 2015 / Published: 10 April 2015
(This article belongs to the Special Issue Mechanics of Biomaterials)
View Full-Text   |   Download PDF [3343 KB, uploaded 10 April 2015]   |  


Hydrogels are used for 3D in vitro assays and tissue engineering and regeneration purposes. For a thorough interpretation of this technology, an integral biomechanical characterization of the materials is required. In this work, we characterize the mechanical and functional behavior of two specific hydrogels that play critical roles in wound healing, collagen and fibrin. A coherent and complementary characterization was performed using a generalized and standard composition of each hydrogel and a combination of techniques. Microstructural analysis was performed by scanning electron microscopy and confocal reflection imaging. Permeability was measured using a microfluidic-based experimental set-up, and mechanical responses were analyzed by rheology. We measured a pore size of 2.84 and 1.69 μm for collagen and fibrin, respectively. Correspondingly, the permeability of the gels was 1.00·10−12 and 5.73·10−13 m2. The shear modulus in the linear viscoelastic regime was 15 Pa for collagen and 300 Pa for fibrin. The gels exhibited strain-hardening behavior at ca. 10% and 50% strain for fibrin and collagen, respectively. This consistent biomechanical characterization provides a detailed and robust starting point for different 3D in vitro bioapplications, such as collagen and/or fibrin gels. These features may have major implications for 3D cellular behavior by inducing divergent microenvironmental cues. View Full-Text
Keywords: wound healing; 3D; in vitro; hydrogel; scaffold; biomechanical; biophysical; microstructure; permeability; rheology wound healing; 3D; in vitro; hydrogel; scaffold; biomechanical; biophysical; microstructure; permeability; rheology

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Moreno-Arotzena, O.; Meier, J.G.; del Amo, C.; García-Aznar, J.M. Characterization of Fibrin and Collagen Gels for Engineering Wound Healing Models. Materials 2015, 8, 1636-1651.

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