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Long-Term Stability of a Cellulose-Based Glucose Oxidase Membrane
National Institute of Advanced Industrial Science and Technology, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan
* Author to whom correspondence should be addressed.
Received: 28 November 2013; in revised form: 22 January 2014 / Accepted: 23 January 2014 / Published: 28 January 2014
Abstract: A cellulose-based glucose oxidase membrane was prepared on a glassy carbon (GC) electrode. The current response of the electrode to glucose was measured by applying a potential of 1.0 V vs. Ag/AgCl on the base GC and was proportional to the concentration of glucose up to 1 mM. The long-term stability of the electrode was examined by measuring the daily glucose response. Over four months, the response magnitude was maintained and then gradually decreased. After 11 months, though the response magnitude decreased to 50% of the initial value, the linear response range did not change. Therefore, the electrode could be used as a glucose biosensor even after 11 months of use. The entrapment of the enzyme in the cellulose matrix promoted the stability of the enzyme, as revealed by data on the enzyme activity after the enzyme electrode was immersed in urea. Therefore, the cellulose matrix may be used to improve the performance of biosensors, bioreactors and bio-fuel cells.
Keywords: cellulose membrane; enzyme electrode; immobilizing enzymes; ionic liquids; long-term stability
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MDPI and ACS Style
Yabuki, S.; Iwamoto, M.; Hirata, Y. Long-Term Stability of a Cellulose-Based Glucose Oxidase Membrane. Materials 2014, 7, 899-905.
Yabuki S, Iwamoto M, Hirata Y. Long-Term Stability of a Cellulose-Based Glucose Oxidase Membrane. Materials. 2014; 7(2):899-905.
Yabuki, Soichi; Iwamoto, Miho; Hirata, Yoshiki. 2014. "Long-Term Stability of a Cellulose-Based Glucose Oxidase Membrane." Materials 7, no. 2: 899-905.