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In Vitro Activity of Gentamicin-Loaded Bioabsorbable Beads against Different Microorganisms
Department of Orthopaedics and Traumatology, Lausanne University Hospital, Rue du Bugnon 46, Lausanne CH-1011, Switzerland
Department of Infectious Diseases, Lausanne University Hospital, Lausanne, CH-1011, Switzerland
Center for Musculoskeletal Surgery, Charité-University Medicine, Free and Humboldt-University of Berlin, Berlin D-10117, Germany
* Author to whom correspondence should be addressed.
Received: 31 May 2013; in revised form: 15 July 2013 / Accepted: 26 July 2013 / Published: 5 August 2013
Abstract: Osteomyelitis is responsible for high treatment costs, long hospital stays, and results in substantial morbidity. Treatment with surgical debridement and antibiotic-impregnated Polymethylmetacrylate (PMMA) beads is the standard of care, providing high local but low serum antibiotic concentrations, thereby avoiding systemic toxicity. However, for several reasons, the beads require surgical removal. Alternative antibiotic delivery systems should improve the treatment of bone infection, actively encourage bone healing and require no additional surgery for removal. We investigated the activity of gentamicin-loaded bioabsorbable beads against different microorganisms (Staphylococcus epidermidis, S. aureus, Escherichia coli, Enterococcus faecalis, Candida albicans) commonly causing surgical site bone infection, by microcalorimetry. Calcium sulphate beads containing gentamicin were incubated in microcalorimetry ampoules containing different concentrations of the corresponding microorganism. Growth medium with each germ and unloaded beads was used as positive control, growth medium with loaded beads alone as negative control. Bacterial growth-related heat production at 37 °C was measured for 24 h. Cultures without gentamicin-loaded beads produced heat-flow peaks corresponding to the exponential growth of the corresponding microorganisms in nutrient-rich medium. In contrast, cultures with gentamicin-loaded beads completely suppressed heat production during 24 h, demonstrating their antibiotic activity. Gentamicin-loaded beads effectively inhibited growth of susceptible microorganisms, under the described in vitro conditions.
Keywords: osteomyelitis; bone graft substitute; antibiotic-loaded bioabsorbable beads; microcalorimetry
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MDPI and ACS Style
Thein, E.; Tafin, U.F.; Betrisey, B.; Trampuz, A.; Borens, O. In Vitro Activity of Gentamicin-Loaded Bioabsorbable Beads against Different Microorganisms. Materials 2013, 6, 3284-3293.
Thein E, Tafin UF, Betrisey B, Trampuz A, Borens O. In Vitro Activity of Gentamicin-Loaded Bioabsorbable Beads against Different Microorganisms. Materials. 2013; 6(8):3284-3293.
Thein, Eric; Tafin, Ulrika F.; Betrisey, Bertrand; Trampuz, Andrej; Borens, Olivier. 2013. "In Vitro Activity of Gentamicin-Loaded Bioabsorbable Beads against Different Microorganisms." Materials 6, no. 8: 3284-3293.