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Materials 2013, 6(12), 5763-5795; doi:10.3390/ma6125763

Semiconductor Nanomaterials-Based Fluorescence Spectroscopic and Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometric Approaches to Proteome Analysis

1
Department of Chemistry, S. V. National Institute of Technology, Surat 395007, India
2
Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
3
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
4
Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
5
Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan
6
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 806, Taiwan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 24 July 2013 / Revised: 14 October 2013 / Accepted: 18 October 2013 / Published: 9 December 2013
(This article belongs to the Section Biomaterials)
View Full-Text   |   Download PDF [917 KB, uploaded 9 December 2013]   |  

Abstract

Semiconductor quantum dots (QDs) or nanoparticles (NPs) exhibit very unusual physico-chemcial and optical properties. This review article introduces the applications of semiconductor nanomaterials (NMs) in fluorescence spectroscopy and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for biomolecule analysis. Due to their unique physico-chemical and optical properties, semiconductors NMs have created many new platforms for investigating biomolecular structures and information in modern biology. These semiconductor NMs served as effective fluorescent probes for sensing proteins and cells and acted as affinity or concentrating probes for enriching peptides, proteins and bacteria proteins prior to MALDI-MS analysis. View Full-Text
Keywords: quantum dots; fluorescent and affinity probes; biomolecules; fluorescence spectroscopy; MALDI-MS quantum dots; fluorescent and affinity probes; biomolecules; fluorescence spectroscopy; MALDI-MS
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Kailasa, S.K.; Cheng, K.-H.; Wu, H.-F. Semiconductor Nanomaterials-Based Fluorescence Spectroscopic and Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometric Approaches to Proteome Analysis. Materials 2013, 6, 5763-5795.

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