Difference between Toxicities of Iron Oxide Magnetic Nanoparticles with Various Surface-Functional Groups against Human Normal Fibroblasts and Fibrosarcoma Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Physicochemical Characterizations of Fe3O4 MNPs
Coating material | Average diameter (nm) | Surface charge (mV) | Functional group |
---|---|---|---|
none (bare) | 10 ± 3 | −20 ± 0.5 | –O− |
TEOS | 100–150 | −30 ± 1.8 | –O− |
APTMS | 10 ± 4 | 25 ± 1.2 | –NH3+ |
TEOS/APTMS | 100–150 | 30 ± 2.0 | –NH3+ |
2.2. Effects of MNPs on Metabolic Activity
2.3. Effects of MNPs on Cell Membrane Integrity
2.4. SEM and TEM Observations
2.5. Effects of MNPs on DNA Stability
3. Experimental Section
3.1. Synthesis and Characterizations of Surface-Functionalized MNPs
3.1.1. Bare MNPs
3.1.2. TEOS-Coated MNPs
3.1.3. APTMS-Coated MNPs
3.1.4. TEOS/APTMS-Coated MNPs
3.1.5. Physicochemical Characterizations
3.2. Cell Cultures and Conditions
3.3. CCK-8 Assay for Cytotoxicity Determination
3.4. LDH Assay for Cytotoxicity Determination
3.5. Electron Microscopy Observations
3.6. Comet Assay for Genotoxicity Evaluation
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Yang, W.J.; Lee, J.H.; Hong, S.C.; Lee, J.; Lee, J.; Han, D.-W. Difference between Toxicities of Iron Oxide Magnetic Nanoparticles with Various Surface-Functional Groups against Human Normal Fibroblasts and Fibrosarcoma Cells. Materials 2013, 6, 4689-4706. https://doi.org/10.3390/ma6104689
Yang WJ, Lee JH, Hong SC, Lee J, Lee J, Han D-W. Difference between Toxicities of Iron Oxide Magnetic Nanoparticles with Various Surface-Functional Groups against Human Normal Fibroblasts and Fibrosarcoma Cells. Materials. 2013; 6(10):4689-4706. https://doi.org/10.3390/ma6104689
Chicago/Turabian StyleYang, Won Jun, Jong Ho Lee, Seong Cheol Hong, Jaewook Lee, Jaebeom Lee, and Dong-Wook Han. 2013. "Difference between Toxicities of Iron Oxide Magnetic Nanoparticles with Various Surface-Functional Groups against Human Normal Fibroblasts and Fibrosarcoma Cells" Materials 6, no. 10: 4689-4706. https://doi.org/10.3390/ma6104689