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Biotechnology Applications of Tethered Lipid Bilayer Membranes
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore
Centre for Biomimetic Sensor Science, 50 Nanyang Drive, Singapore 637553, Singapore
Austrian Institute of Technology (AIT) GmbH, Donau-City Str.1, Vienna 1220, Austria
* Author to whom correspondence should be addressed.
Received: 8 October 2012; in revised form: 23 November 2012 / Accepted: 26 November 2012 / Published: 7 December 2012
Abstract: The importance of cell membranes in biological systems has prompted the development of model membrane platforms that recapitulate fundamental aspects of membrane biology, especially the lipid bilayer environment. Tethered lipid bilayers represent one of the most promising classes of model membranes and are based on the immobilization of a planar lipid bilayer on a solid support that enables characterization by a wide range of surface-sensitive analytical techniques. Moreover, as the result of molecular engineering inspired by biology, tethered bilayers are increasingly able to mimic fundamental properties of natural cell membranes, including fluidity, electrical sealing and hosting transmembrane proteins. At the same time, new methods have been employed to improve the durability of tethered bilayers, with shelf-lives now reaching the order of weeks and months. Taken together, the capabilities of tethered lipid bilayers have opened the door to biotechnology applications in healthcare, environmental monitoring and energy storage. In this review, several examples of such applications are presented. Beyond the particulars of each example, the focus of this review is on the emerging design and characterization strategies that made these applications possible. By drawing connections between these strategies and promising research results, future opportunities for tethered lipid bilayers within the biotechnology field are discussed.
Keywords: tethered lipid bilayer; supported lipid membranes; ion channel; stochastic sensing; cytochrome c; electrochemical impedance spectroscopy; fluorescence recovery after photobleaching; surface plasmon resonance; quartz crystal microbalance; two-dimensional surface-enhanced IR absorption spectroscopy
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Cite This Article
MDPI and ACS Style
Jackman, J.A.; Knoll, W.; Cho, N.-J. Biotechnology Applications of Tethered Lipid Bilayer Membranes. Materials 2012, 5, 2637-2657.
Jackman JA, Knoll W, Cho N-J. Biotechnology Applications of Tethered Lipid Bilayer Membranes. Materials. 2012; 5(12):2637-2657.
Jackman, Joshua A.; Knoll, Wolfgang; Cho, Nam-Joon. 2012. "Biotechnology Applications of Tethered Lipid Bilayer Membranes." Materials 5, no. 12: 2637-2657.