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Physisorbed Polymer-Tethered Lipid Bilayer with Lipopolymer Gradient
Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis, 402 N Blackford St., Indianapolis, IN 46202, USA
Integrated Nanosystems Development Institute, Indiana University-Purdue University Indianapolis, 755 W Michigan St., Indianapolis, IN 46202, USA
* Author to whom correspondence should be addressed.
Received: 15 October 2012; in revised form: 6 November 2012 / Accepted: 6 November 2012 / Published: 8 November 2012
Abstract: Physisorbed polymer-tethered lipid bilayers consisting of phospholipids and lipopolymers represent an attractive planar model membrane platform, in which bilayer fluidity and membrane elastic properties can be regulated through lipopolymer molar concentration. Herein we report a method for the fabrication of such a planar model membrane system with a lateral gradient of lipopolymer density. In addition, a procedure is described, which leads to a sharp boundary between regions of low and high lipopolymer molar concentrations. Resulting gradients and sharp boundaries are visualized on the basis of membrane buckling structures at elevated lipopolymer concentrations using epifluorescence microscopy and atomic force microscopy. Furthermore, results from spot photobleaching experiments are presented, which provide insight into the lipid lateral fluidity in these model membrane architectures. The presented experimental data highlight a planar, solid-supported membrane characterized by fascinating length scale-dependent dynamics and elastic properties with remarkable parallels to those observed in cellular membranes.
Keywords: gradient bilayer; Langmuir-Blodgett transfer; lipopolymer; polymer-tethered lipid membrane; Epifluorescence; atomic force microscopy; buckling structure
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MDPI and ACS Style
Lin, Y.-H.; Minner, D.E.; Herring, V.L.; Naumann, C.A. Physisorbed Polymer-Tethered Lipid Bilayer with Lipopolymer Gradient. Materials 2012, 5, 2243-2257.
Lin Y-H, Minner DE, Herring VL, Naumann CA. Physisorbed Polymer-Tethered Lipid Bilayer with Lipopolymer Gradient. Materials. 2012; 5(11):2243-2257.
Lin, Yu-Hung; Minner, Daniel E.; Herring, Vincent L.; Naumann, Christoph A. 2012. "Physisorbed Polymer-Tethered Lipid Bilayer with Lipopolymer Gradient." Materials 5, no. 11: 2243-2257.