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Materials 2011, 4(8), 1360-1374; doi:10.3390/ma4081360
Article

Osteoinductivity Assessment of BMP-2 Loaded Composite Chitosan-Nano-Hydroxyapatite Scaffolds in a Rat Muscle Pouch

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Received: 30 June 2011; in revised form: 16 July 2011 / Accepted: 27 July 2011 / Published: 2 August 2011
(This article belongs to the Special Issue Carbohydrate Polymers)
Download PDF [978 KB, uploaded 2 August 2011]
Abstract: The objective of this study was to evaluate the osteoinductivity of composite chitosan-nano-hydroxyapatite scaffolds in a rat muscle pouch model. Previous in vitro characterization demonstrated the ability of the scaffolds to promote bone regeneration and as a carrier for local delivery of BMP-2. Composite microspheres were prepared using a co-precipitation method, and scaffolds were fabricated using an acid wash to adhere beads together. To determine the in vivo osteoinductivity of the scaffolds, the following groups (n = 6) were implanted into muscle pouches created in the latissimus dorsi of Sprague Dawley rats: (A) lyophilized scaffolds without rhBMP-2, (B) lyophilized scaffolds with rhBMP-2, (C) non-lyophilized scaffolds with rhBMP-2, and (D) absorbable collagen sponge with rhBMP-2 (control). Groups B, C, and D were loaded with 4 mL of a 9.0 μg/mL solution of rhBMP-2 for 48 h. The rats were sacrificed after one month and samples were analyzed for amount of residual implant material, new bone, and osteoid. Although the experimental groups displayed minimal degradation after one month, all of the scaffolds contained small amounts of woven bone and considerable amounts of osteoid. Approximately thirty percent of the open space available for tissue ingrowth in the scaffolds contained new bone or osteoid in the process of mineralization. The ability of the composite scaffolds (with and without BMP-2) to promote ectopic bone growth in vivo was demonstrated.
Keywords: chitosan; osteoinductivity; hydroxyapatite; BMP-2; animal study chitosan; osteoinductivity; hydroxyapatite; BMP-2; animal study
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Reves, B.T.; Jennings, J.A.; Bumgardner, J.D.; Haggard, W.O. Osteoinductivity Assessment of BMP-2 Loaded Composite Chitosan-Nano-Hydroxyapatite Scaffolds in a Rat Muscle Pouch. Materials 2011, 4, 1360-1374.

AMA Style

Reves BT, Jennings JA, Bumgardner JD, Haggard WO. Osteoinductivity Assessment of BMP-2 Loaded Composite Chitosan-Nano-Hydroxyapatite Scaffolds in a Rat Muscle Pouch. Materials. 2011; 4(8):1360-1374.

Chicago/Turabian Style

Reves, Benjamin T.; Jennings, Jessica A.; Bumgardner, Joel D.; Haggard, Warren O. 2011. "Osteoinductivity Assessment of BMP-2 Loaded Composite Chitosan-Nano-Hydroxyapatite Scaffolds in a Rat Muscle Pouch." Materials 4, no. 8: 1360-1374.


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